BLAST序列比对脱机移植研究

Research on Offline Environment of BLAST Sequence Alignment

BLAST序列比对算法是NCBI综合性生物信息平台整合的众多重要功能之一。研究建立BLAST算法的脱机环境移植,可使生物信息学研究人员在构建自己专有序列数据库的同时,对DNA序列进行脱机环境比对,以期更高的序列数据安全性,避免联网状态下造成的数据丢失和泄露等严重问题。通过研究BLAST序列比对过程中涉及的标准数据格式与调用细节,实现了脱机环境下的BLAST序列比对,为建立安全序列比对提供了一种可行参考。

The BLAST is one of the many important functions integrated by the NCBI comprehensive bioinformatics platform.Researching the offline environment of BLAST help bioinformatics researchers build their own proprietary sequence database and do BLAST in Offline environment,so that data corruption or data leakage which caused by the network connection state effectively are avoided and the sequence data provided is in higher security guarantee.The research studied the standard data format and call details involved in the BLAST sequence alignment process,made the BLAST sequence alignment in offline environment actualized,and established a feasible reference for establishing a secure sequence alignment.