壳聚糖/功能性自组装多肽复合支架的制备及神经再生研究

Preparation of Chitosan/Functional Self-Assembling Peptide Composite Scaffolds for Nerve Regeneration

利用天然生物交联剂京尼平交联壳聚糖(CS)和功能性自组装多肽(F-SAP),经冷冻干燥制备了不同配比的CS/F-SAP复合支架;通过比较复合支架的微观形貌、孔隙率、力学性能和吸水率确定复合支架的最佳配比,并通过研究复合支架对神经干细胞分化的影响探究其对神经再生的作用。结果表明,CS/F-SAP复合支架具有多孔结构;20 mg·mL^-1的CS溶液与10 mg·mL^-1F-SAP溶液最佳体积比为1∶1,该条件下制备的CS/F-SAP复合支架能够促进神经干细胞向神经元方向分化,促进神经再生。

Through the cross-linking of chitosan(CS)and functional self-assembling peptide(F-SAP)with a natural bio-crosslinker Genipin,we prepared CS/F-SAP composite scaffolds with different proportions by freeze-drying.By comparing the micro morphology,porosity,mechanical property,and water absorption of the composite scaffolds,we optimized the proportion of the composite scaffolds.Moreover,we investigated the role of the composite scaffolds in nerve regeneration by studying the effect of the composite scaffolds on the differentiation of neural stem cells.The results show that the CS/F-SAP composite scaffold has a porous structure,and the optimal volume ratio of 20 mg·mL^-1CS solution to 10 mg·mL^-1F-SAP solution is 1∶1.The CS/F-SAP composite scaffold prepared under this condition can promote the differentiation of neural stem cells into neurons,thus promoting nerve regeneration.