响应面法优化苯酚-硫酸法测定泡桐花多糖含量

Optimization in Phenol-Sulfuric Acid Method for Determination of Content of Paulowniae flos Polysaccharide by Response Surface Methodology

对苯酚-硫酸法测定泡桐花多糖含量进行方法学验证,采用单因素实验考察苯酚用量、硫酸用量、水浴温度、显色时间等对泡桐花多糖含量测定的影响,采用响应面法对苯酚-硫酸法的测定条件进行优化,并在最佳条件下对泡桐花多糖含量进行测定。结果表明,葡萄糖浓度在0.01~0.08 mg·mL^-1范围内与吸光度线性关系良好(R=0.9993),泡桐花多糖加标回收率在98.94%~101.93%之间,RSD在1.0%~1.4%之间。苯酚-硫酸法测定泡桐花多糖含量的最佳条件为:苯酚用量0.4 mL、硫酸用量5.0 mL、显色时间25 min,在此条件下,泡桐花多糖平均含量为2.65%,RSD为2.95%。该方法稳定可靠、精密度好、准确度高,可用于泡桐花多糖的含量测定。

We performed the methodology validations for the determination of the content of Paulowniae flos polysaccharide by a phenol-sulfuric acid method.Moreover,we investigated the effects of phenol amount,sulfuric acid amount,water bath temperature,and coloration time on the determination of the content of Paulowniae flos polysaccharide by single-factor experiments,and then further optimized the determination conditions of phenol-sulfuric acid method by response surface methodologies.Furthermore,we determined the content of Paulowniae flos polysaccharide under the optimal conditions.The results show that the linear relationship is good in the range of 0.01-0.08 mg·mL^-1(R=0.9993),the adding standard recovery of Paulowniae flos polysaccharide is 98.94%-101.93%,and the RSD is 1.0%-1.4%.The optimal conditions are determined as follows:the phenol amount of 0.4 mL,the sulfuric acid amount of 5.0 mL,and the coloration time of 25 min.Under above conditions,the average content of Paulowniae flos polysaccharide is 2.65%,and the RSD is 2.95%.The optimized method is stable and reliable with good precision and high accuracy,which can be used for the determination of the content of Paulowniae flos polysaccharide.