毛木耳A交配型位点的克隆和序列结构分析

Cloning and sequence structure analysis of A mating type loci of Auricularia cornea

以毛木耳Auricularia cornea杂交子APM2-16的亲本单核体APP7和M2S16为研究材料,经同源序列比对,从单核体APP7的基因组中获得了A交配型位点序列,并定位了mip基因的位置。根据APM2-16的转录组和单核体APP7基因组的相关信息设计引物,通过PCR扩增和克隆测序的方式获得单核体APP7和M2S16中的HD基因序列,通过生物信息学分析发现两者在核苷酸序列上没有同源性,都编码同源结构域转录因子。通过对毛木耳交配型位点的研究有助于进一步开展该物种的遗传学基础研究,为菌种遗传改良奠定基础。

Using the parental monokaryons APP7 and M2S16 of the Auricularia cornea hybrid APM2-16 as research materials,the sequence of A mating type loci was obtained from the genome of the monokaryon APP7 by homologous sequence alignment,and the physical location of mip was mapped.Primers were designed according to the transcriptome of APM2-16 and the related information of the monokaryon APP7 genome.The HD gene sequences in monokaryons APP7 and M2S16 were obtained by PCR amplification and sequencing.It was revealed that the two had no homology in the nucleotide sequences,and both encoded homodomain transcription factors through bioinformatics analysis.This study is helpful to spawn genetic improvement of A.cornea.