金针菇交配型B第三个亚位点的发现与遗传

The discovery and inheritance of the third mat-B sublocus in Flammulina filiformis

本研究在分析金针菇减数分裂重组热点时发现B交配型位点可能存在第三个亚位点,为此对同核菌株’6-3’和’6-21’的B交配型位点进行全基因组注释,获得8对同源信息素受体基因(pheromone receptor gene,pr)和3对同源信息素前体基因(pheromone precursor gene,pp),组成了2个结构完整、相距326kb的亚位点α和β;’6-21’菌株还发现1个’6-3’菌株不具有的pr,组成第3个亚位点γ,它与亚位点α和β没有连锁。’6-3’和’6-21’配对形成异核体后出菇,分离获得142个单孢菌株,其B交配型有8种基因型,通过亲和性试验得到证实,同时还验证了第3个亚位点γ具有与α、β相同的功能。

During analysis of the meiotic recombination hotspots,we speculated that there might be a third mat-B sublocus presenting in the genome of Flammulina filiformis.The genomes of the homokaryotic strains‘6-3’and‘6-21’were annotated,and 8 paired homologous pheromone receptor genes and 3 paired homologous pheromone precursor genes were found,which constituted two mat-B subloci(αandβ)those were 326kb apart.An extra pheromone precursor gene was found in the strain‘6-21’,which was absent in‘6-3’,constituting the third mat-B sublocusγunlinked withαandβ.Heterocaryon formed by mating‘6-3’with‘6-21’was cultivated for fruiting,and 142 single spore strains were isolated from the fruit-bodies.As a result,there were 8 genotypes of mat-B confirmed by compatibility tests.The third sublocus of mat-B was verified to have the same function asαandβloci.