低强度脉冲电磁场加速2型糖尿病表皮软组织创伤愈合实验

Low-intensity pulsed electromagnetic fields accelerates skin wound healing in type 2 diabetic mice:an experimental research

目的:研究低强度的脉冲电磁场(PEMF)对于2型糖尿病软组织创伤愈合的治疗效果。方法:32只3月龄雄性2型糖尿病db/db小鼠及16只同背景的野生对照小鼠用于本研究,分为Wild type野生型小鼠组(Wild type)、db/db小鼠组(db/db)及db/db施加以PEMF刺激组(db/db+PEMF),每组16只。分别于创伤模型构建的第5、12、19天处死每组的4只小鼠。全部小鼠均构建背部表皮创伤模型,db/db+PEMF组小鼠施以全身PEMF刺激,每天刺激2 h。分别通过伤口闭合率、伤口组织抗张强度、伤口组织病理和ELISA检测分析评估其作用效果。结果:PEMF刺激在术后的第5、12、19天均显著提升了db/db小鼠伤口的愈合速率(P<0.05),降低了db/db小鼠的伤口总愈合时间(P<0.05),在术后的第5、12、19天均显著提升了伤口组织的抗张强度(P<0.05),降低了伤口组织的炎性细胞数量且显著降低了炎性因子IL-1β和TNF-α的表达(P<0.05),并上调了VEGF和IGF-1的表达(P<0.05)。结论:低强度PEMF能够显著加速2型糖尿病db/db小鼠的软组织修复进程,这一效应可能与PEMF的抗炎性反应及促胶原沉积和血管再生相关。

Objective To identify the therapeutic effects of low-intensity pulsed electromagnetic fields(PEMF)on the soft tissue wound healing in type 2 diabetic mice.Methods Thirty-two 3-month-old male type 2 diabetic db/db mice and 16 wild type control mice with the same background were assigned to db/db group(db/db)and db/db treated with PEMF group(db/db+PEMF)and wild type group(Wild type),with 16 mice in each group.Four mice in each group were sacrificed on day 5,12 and 19 after the wound model establishment.The induction of dorsal wound model was performed on all mice.The mice in db/db+PEMF group were subjected to whole-body PEMF stimulation for 2 h per day.The therapeutic effects were evaluated by wound healing rate,wound tensile strength,wound tissue histopathology and ELISA assay.Results PEMF stimulation significantly increased the wound healing rate in db/db mice on the 5th,12nd and 19th day postoperatively(P<0.05),and also decreased the overall wound healing period in db/db mice(P<0.05).Moreover,PEMF simulation increased wound tensile strength on the 5th,12nd and 19th day postoperatively(P<0.05),and also decreased the number of inflammatory cells in wound tissues,suppressed the expressions of inflammatory cytokines IL-1βand TNF-α(P<0.05),increased the expressions of VEGF and IGF-1(P<0.05).Conclusion Low-intensity PEMF stimulation is capable of significantly accelerating soft tissue healing in type 2 diabetic db/db mice,which may be related to its anti-inflammatory response and promotion of collagen deposition and vascular regeneration.