超高效液相色谱串联质谱法测定饮用水中双酚S、双酚F和双酚A

Direct Determination of Bisphenol S,Bisphenol F,and Bisphenol A in Drinking Water by Ultra-performance Liquid Chromatography-Mass Spectrometry

目的建立饮用水中双酚S(BPS)、双酚F(BPF)和双酚A(BPA)的超高效液相色谱—串联质谱(UPLC-MS/MS)直接测定方法。方法以甲醇—水为流动相梯度洗脱,水样经Waters ACQUITY UPLCHSS T3色谱柱分离,采用负离子电喷雾多反应监测模式进行检测,外标法定量。结果UPLC-MS/MS色谱分离良好,BPS、BPF和BPA在(0.5~50.0)μg/L浓度范围内线性相关系数均大于0.997,BPS、BPF和BPA的方法检出限分别为0.002、0.05和0.15μg/L,加标回收率范围为90.1%~108%,相对标准偏差(RSD)为0.57%~5.90%。结论UPLC-MS/MS操作简便、分析速度快、灵敏度高,适用于饮用水中双酚类化合物的测定。

Objective To establish an ultra-performance liquid chromatography-mass spectrometry method for direct determination of bisphenol S(BPS),bisphenol F(BPF),and bisphenol A(BPA)in drinking water.Methods With a mobile phase of methanol-water for gradient elution,the water samples were separated on a Waters Acquity UPLC-HSS T3 column(2.1 mm×100 nm,1.8μm)and were then detected by electrospray ionization mass spectrometry in multi-reaction monitoring mode,and the external standard method was used for quantification.Results This method had good chromatographic separation,and BPS,BPF,and BPA had a linear correlation coefficient of>0.997 within the range of(0.05~50.0)μg/L.The method detection limits of BPS,BPF,and BPA were 0.002,0.05,and 0.15μg/L,respectively,and the recovery of standard addition ranged from 90.1%to108%,with relative standard deviations of 0.57%~5.90%.Conclusion The method is simple,rapid,and sensitive and can be used for determining bisphenol compounds in drinking water.