氮素对枸杞甜菜碱及其代谢关键酶的影响

Effects of Different Nitrogen Application Treatments on Betaine and Its Metabolic Key Enzymes

【目的】本试验通过科学设计、实施及相关指标测定,明确氮素与宁夏枸杞甜菜碱及代谢关键酶间关系,为宁夏枸杞品质化栽培提供理论依据。【方法】采用三因素随机区组试验设计,以宁杞1号为试材,设氮素施氮深度、基追比例、施氮量水平三个因素,研究氮素对宁夏枸杞不同生长时期甜菜碱含量及其代谢关键酶的影响。【结果】氮肥施用深度为0~20 cm时,枸杞甜菜碱、甜菜碱醛脱氢酶、胆碱单加氧酶含量及产量最高,随着深度的增加,枸杞甜菜碱及2种酶含量逐渐下降;甜菜碱含量在结果前基追比为3∶1时最高,结果后2∶1∶1时最高;甜菜碱醛脱氢酶与胆碱单加氧酶含量整体在基追比例为3∶1时含量最高;随着施氮量的增加,甜菜碱及2种酶的含量呈下降趋势,而产量则呈上升趋势,枸杞产量在施氮量40 kg/667m^2时最适,比不施肥及施氮30、50 kg/667m^2分别高出88.13%、29%、3%。【结论】合理氮肥施用可明显提高枸杞产量,改善枸杞品质。综合分析,氮素在施氮量40 kg/667m^2,施氮深度0~20 cm时对产量、甜菜碱及其代谢关键酶含量累积有促进作用,氮肥基追比例为3∶1时对促进2种酶含量累积效果明显。

【Objective】In this experiment,the relationship between nitrogen and betaine and key enzymes of Ningxia Lycium barbarum by scientific design,implementation and related indicators determination,was determined,which provided a theoretical basis for the quality cultivation of Ningxia wolfberry.【Method】In this experiment,three factors randomized block design was used to study Ningqi 1 astested material.The effects of nitrogen on the content of betaine and the key enzymes of metabolism in Ningxia Lycium barbarum at different growth stages were studied based on three factors including nitrogen depth,basal dressing ratio and nitrogen level.【Result】The content and yield of betaine,betaine aldehyde dehydrogenase and choline monooxygenase of Lycium barbarum were the highest when the depth of nitrogen fertilizer application was 0-20 cm.With the increase of depth,the content of betaine and two enzymes of Lycium barbarum decreased gradually.The content of betaine was the highest when the basal ratio was 3∶1,and the highest was at 2∶1∶1.The contents of betaine aldehyde dehydrogenase and choline monooxygenase were the highest when the basal ratio was 3∶1.With the increase of nitrogen application rate,the content of betaine and two enzymes decreased,while the yield increased.The yield ofLycium barbarum was the most suitable at 40 kg/667 m^2,which was 88.13 %,29 %,3 % higher than that of no fertilization and 30 and 50 kg/667 m^2,respectively.【Conclusion】Rational application of nitrogen fertilizer can significantly improve the yield and quality ofLycium barbarum.Comprehensive analysis showed that the nitrogen application at 40 kg/667 m^2 and 0-20 cm depth promoted the accumulation of yield,betaine and key metabolic enzymes,and the accumulation of two enzymes was obvious at the ratio of base topdressing to top dressing at 3∶1.