摘要
【目的】本文研究了霉酚酸对PPRV感染的抑制作用。【方法】采用不同浓度的霉酚酸处理Vero细胞,运用显微镜观察和q-PCR方法,研究霉酚酸的抗病毒作用;通过额外补充鸟苷酸,探究霉酚酸的抗PPRV作用机理。【结果】霉酚酸能够抑制PPRV的复制,最佳作用浓度为1μmol/L;霉酚酸抗PPRV的最佳作用时间为96 h;额外添加鸟苷酸发现,鸟苷酸可拮抗霉酚酸的抗PPRV作用,霉酚酸可能通过抑制细胞内鸟苷酸的合成来抑制PPRV的复制。【结论】研究了霉酚酸的抗PPRV作用,以期为PPRV的防控提供参考。
【Objective】This study aimed to investigate the effect of mycophenolic acid on PPRV infection.【Method】Vero cells were treated with different concentrations of mycophenolic acid,and the antiviral effect of mycophenolic acid was studied by microscope observation and q-PCR method,the mechanism of anti-PPRV effect of mycophenolicacid by adding guanosine.【Result】Themycophenolic acid could inhibit the replication of PPRV,the optimum concentration of mycophenolic acid was 1μmol/L,and the best action time of mycophenolic acid against PPRV was 96 hours.It was found that guanosine could antagonize the anti-PPRV effect of mycophenolic acid,and mycophenolic acid might inhibit the replication of PPRV by inhibiting the synthesis of guanosine in cells.【Conclusion】In this paper,the anti-PPRV effect of mycophenolic acid was studied in order to provide a reference for the prevention and control of PPRV.
作者
张邵博
摆倩文
常秋燕
丁功涛
皮尔·穆罕默德·阿卜杜勒
ZHANG Shao-bo;BAI Qian-wen;CHANG Qiu-yan;DING Gong-tao;PEER Mohamed Abdul(Biomedical Research Center,Northwest Minzu University,Gansu Lanzhou 730030,China;Life Science and Engineering College of Northwest University for Nationalities,Gansu Lanzhou 730030,China;Faculty of Engineering&Built Environment,Universitig Kebangsaan Malaysia,Bangi,Selangor 43600,Malaysia)
出处
《西南农业学报》
CSCD
北大核心
2020年第6期1314-1318,共5页
Southwest China Journal of Agricultural Sciences
基金
甘肃省国际科技合作项目(17YF1WA166)
科技部科技伙伴计划(KY201501005)
西北民族大学中央高校基本科研业务费专项资金资助研究生项目(Yxm2019145)。
关键词
小反刍兽疫病毒
霉酚酸
鸟苷酸
抗病毒
Peste des petitsruminantsvirus
Mycophenolic acid
Guanosine
Antivirus
