发光铜基金属有机骨架在四环素类抗生素检测中的应用研究

Study of Luminescent Cu-MOF and Its Application in Detecting Tetracyclines

利用发光铜基金属有机骨架(Cu-MOF)纳米材料构建了一种检测四环素的新方法.Cu-MOF纳米材料由水热法一步合成,具有规则的片状结构,片状结构进而堆积排列成花状结构.加入四环素后,Cu-MOF纳米材料的荧光被淬灭,进一步实验证明荧光内滤效应在该淬灭中起主要作用.该方法的线性检测范围为0-60μM,检出限为33.33 nM,低于中国、美国和欧盟的标准.此外,本方法选择性好,常见的其他抗生素和金属离子均不能产生干扰.最后,我们对当地河水进行四环素检测,加标回收率为96.17%-105.02%.

In this contribution,a new method for detection of tetracyclines is constructed by employing luminescent copper-based metal organic frameworks(Cu-MOF)nanomaterials.The Cu-MOF nanomaterials were synthesized by one-step hydrothermal method,showing nano-sheet flower-like structure.The fluorescence of Cu-MOF nanoflowers decreased when tetracyclines were added,and it was experimentally demonstrated the fluorescence quenching was mainly caused by inner-filter effect.The proposed method shows a linear response to tetracyclines in 0-60μM,and the limit of detection is 33.33 nM,which is lower than the standards of China,the US and the Eu.In addition,this method also exhibited good selectivity in the presence of common antibiotics and interfering cations.To further evaluate the analytical performance of this method,tetracyclines were determined in local water samples,and the obtained recovery rate is 96.17%-105.02%.