响应面法优化地骨皮总生物碱微波提取工艺研究

Response Surface Method was used to Optimize the Microwave Extraction Process of Total Alkaloids from Lycii Cortex

目的:以响应面法优化地骨皮总生物碱微波提取工艺。方法:以地骨皮中总生物碱的提取率为考察指标,利用BOX-Benhnken设计-响应面法优化地骨皮总生物碱的微波提取工艺,利用最优工艺条件测定了30批次地骨皮中总生物碱的含量。结果:地骨皮总生物碱最佳提取工艺条件为液料比27∶1(mL∶g),乙醇浓度70%,微波提取温度73℃,微波提取时间22min,在此条件下总生物碱提取率为3.86%。30批地骨皮总生物碱含量在14.68~39.20mg/g之间,安徽、河南、河北所产地骨皮质量稳定,甘肃所产质量差异较大。结论:优化的地骨皮总生物碱微波提取工艺的方法合理、稳定、可行,为地骨皮总生物碱提取方法提供实验依据。

Objective:To optimize the microwave extraction process of total alkaloids from Lycii Cortex by response surface method.Methods:Using Box-Benhnken design-response surface method to optimize the microwave extraction process of total alkaloids from Lycii Cortex,the content of total alkaloids in Lycii Cortex of 30 batches was determined.Results:The optimal extraction conditions of total alkaloids from the Lycii Cortex were:liquid-solid ratio 27∶1(mL∶g),ethanol concentration 70%,microwave extraction temperature 73℃,microwave extraction time 22 min,and total alkaloids extraction rate 3.86%.The total alkaloid content in the Lycii Cortex of 30 batches was 14.68~39.20 mg/g,and the quality of Lycii Cortex from Anhui,Henan and Hebei provinces is stable,and the quality produced by Gansu is quite different.Conclusion:The optimized method of microwave extraction of total alkaloids from Lycii Cortex is reasonable,stable and feasible,which provides experimental basis for the extraction of total alkaloids from Lycii Cortex.