大豆肽对果蝇睡眠的影响

Effects of Soybean Peptide on Sleep in Drosophila

研究大豆肽(soybean peptides,SBP)对果蝇睡眠的影响。通过大豆分离蛋白酶解,制得SBP,选择20日的雄性果蝇,分为4组,无添加的培养基作为空白组,SBP分为2、4、8 mg/mL额外添加到培养基作为3个实验模型组,通过VideoTrack动物行为分析系统监测果蝇睡眠时长,并检测SBP对果蝇脑部生物钟基因(per、tim、clk、cyc)与参与调控睡眠神经递质合成限速酶的基因(gad、tph、gdh、hdc)以及五羟色胺(5-hydroxytryptamine,5-HT)的影响。研究发现,在监测睡眠的第2日晚上,SBP对果蝇睡眠延长效果最好,并呈现剂量依赖性,又通过剥离取头,分析其生物钟基因(per、tim、clk、cyc)和神经递质合成限速酶基因(gad、tph、gdh、hdc),得到SBP是通过调节tim和tph的基因表达并提高5-HT在脑部的含量来延长果蝇的睡眠时长的结论。

To study the effect of soybean peptides(SBP) on sleep in Drosophila,SBP was prepared by proteolytic separation of soybean.20 days-old male Drosophilas melangogaster were selected and divided into 4 groups.The basic medium was used as a blank control group,and three experimental groups were cultured using basic medium containing 2,4 and 8 mg/mL SBP,respectively.The sleep duration was detected by the VideoTrack animal behavior analysis system,and the effects of SBP on circadian clock genes(per,tim,clk,cyc) of Drosophila brain,sleep related genes of rate-limiting enzymes of neurotransmitter synthetase(gad,tph,gdh,hdc),and serotonin(5-HT) were analyzed.The results showed that SBP had the best effect on prolongation of sleep in a dose dependent manner on the second day of sleep monitoring.By stripping the head,the circadian clock genes(per,tim,clk,cyc) and neurotransmitter synthetase genes(gad,tph,gdh,hdc) were analyzed.It was found that SBP administration prolonged the sleep duration of Drosophila melanogaster by up-regulating the levels of tim and tph and increasing the secretion of 5-HT in the brain.