摘要
目的基于网络药理学探讨心脑宁胶囊治疗冠状动脉粥样硬化性心脏病(coronary atherosclerotic heart disease,CHD)的作用机制。方法利用中药数据库、疾病数据库搜索心脑宁胶囊治疗CHD的靶点,并将相关靶点在DAVID数据库进一步富集分析,分析心脑宁胶囊治疗CHD的作用机制,利用Cytoscape构图,利用分子对接技术验证靶点与小分子的结合度。结果共得到95个心脑宁胶囊治疗CHD治疗的作用靶点,富集分析其生物过程与细胞缺氧的反应、炎症反应最为密切,KEGG分析肿瘤坏死因子(TNF)信号通路最为显著,靶点TNF-α与山奈酚有强烈的结合活性。结论基于网络药理学预测出心脑宁胶囊治疗CHD具有多成分-多靶点-多通路的特点,为心脑宁胶囊的实验研究、临床应用提供了理论依据。
Objective To study on the mechanism of Xinnaoning Capsule in treating coronary atherosclerotic heart disease(CHD)based on network pharmacology.Methods Searching the targets of Xinnaoning Capsule for the treatment of CHD through the Chinese Medicine Database(TCMSP)and disease database,and these targets were enriched and analyzed in the DAVID database,the mechanism of action of Xinnaoning Capsule in the treatment of coronary CHD was analyzed using Cytoscape for drawing,the binding degree between targets and small molecule by molecular docking technology was verified.Results There were 95 action targets in the treatment of CHD by Xinnaoning Capsule.In the enrichment analysis,the reaction and inflammatory response of the biological process cells were most significant.The TNF signaling pathway was most prominent in the KEGG analysis.Tumor necrosis factor-alpha had an active binding activity with kaempferol.Conclusion Based on network pharmacology,it is predicted the Xinnaoning Capsule for the treatment of coronary heart disease is characterized by multi-components,multi-targets and multi-pathways,so it can provide a theoretical basis for the experimental study of Xinnaoning Capsule.
作者
李婧
张颖
梅俊
张萍
徐凤芹
LI Jing;ZHANG Ying;MEI Jun;ZHANG Ping;XU Feng-qin(Department of General Internal Medicine,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China)
出处
《北京中医药》
2020年第4期311-316,395,396,共8页
Beijing Journal of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(81774143)。
