基于系统生物学分析防风治疗中风病中经络的作用机制

Mechanism of Radix saposhnikoviae in treating stroke of attack of collateral based on systematic biology

目的基于对中国古代方剂的挖掘,探索防风治疗中风病中经络的作用机制。方法检索中国中医科学院中医药信息研究所提供的中国方剂数据库有关防风治疗中风病中经络的方剂。中风病靶点信息来源于GeneCards数据库。采用频数分析挖掘方剂数据库,选取频次较高药物,借助BATMAN-TCM系统进行系统生物学分析。结果治疗中风病中经络的方剂以防风用药频次最高;选取防风进行系统生物学预测,结果显示防风中28个化合物可通过作用于153个靶点治疗中风病,关键靶点集中在钠离子通道及乙酰胆碱相关受体亚基上,生物信息学分析结果显示其主要功能为调控离子通道或神经配体活性。结论防风通过调控离子通道从而影响神经元电压门控,起到神经修复作用;或通过调控胆碱能受体活性,影响神经免疫功能,延缓脑损伤进程。

Objective To explore the mechanism of Radix saposhnikoviae in treating stroke of attack of collateral based on mining the rules in ancient TCM prescriptions.Methods TCM prescriptions for stroke of attack of collateral were retrieved in China Prescription Database of the Institute on Information of Traditional Chinese Medicine,China Academy of Chinese Medical Sciences from 10 June 2019 to 15 June 2019. Stroke target information came from GeneCards database. The highest frequency herbs were selected and submitted to BATMAN-TCM system to make system biology analysis by mining the prescription database using frequency analysis. Results The frequency of Radix saposhnikoviae was the highest among the drugs for stroke of attack of collateral. So Radix saposhnikoviae was used for prediction in system biology. The result suggested that 28 components of Radix saposhnikoviae could act on a total of 153 target in treating stroke of attack of collateral, and the key targets were concentrated on SCN and CHRNA. Bioinformatics analysis showed that the main function of them was to regulate ion channel or neuroligand activity. Conclusion Radix saposhnikoviae can affect neuronal voltage gating by regulating ion channels to achieve nerve repair;or by regulating the activity of cholinergic receptors, to impact the nerve immune function and delay the process of brain injury.