LASS2/TMSG-1、TAK1和TAB1在结肠癌中的表达及临床意义

Expression and Clinical Significance of LASS2/TMSG-1,TAK1 and TAB1 in Colon Cancer

[目的]探讨肿瘤转移抑制基因-1(LASS2/TMSG-1)、转化生长因子激活激酶1(TAK1)和转化生长因子β活化激酶结合蛋白1 (TAB1)在结肠癌组织中的表达及其临床意义。[方法]选取2017年1月至2018年2月在我院治疗的原发性结肠癌患者68例,分别取其结肠癌组织及癌旁正常组织,采用实时聚合酶联法(real-time polymerase chain reaction,RT-PCR)测定组织中的LASS2/TMSG-1、TAK1、TAB1表达水平,分析结肠癌组织中LASS2/TMSG-1、TAK1、TAB1的表达水平以及与患者临床病理特征的相关性,采用COX回归分析分析LASS2/TMSG-1、TAK1、TAB1表达水平与患者预后的相关性。[结果]结肠癌患者结肠癌组织TAK1(0.76±0.15ng/ml)、TAB1(1.46±0.20ng/ml)的表达水平与癌旁正常组织(0.57±0.15ng/ml,1.21±0.19ng/ml)相比显著升高;LASS2/TMSG-1的表达水平(1.52±0.36ng/ml)与癌旁正常组织(2.36±0.43ng/ml)相比显著下降(P<0.05)。LASS2/TMSG-1、TAK1、TAB1表达水平与肿瘤分化程度、TNM分期显著相关(P<0.05)。术后12个月随访时共有8例(11.76%)患者出现肿瘤复发/转移。出现肿瘤复发/转移的患者其TAK1、TAB1水平(0.96±0.15ng/ml,1.68±0.19ng/ml)与未复发/转移(0.52±0.13ng/ml,1.26±0.20ng/ml)患者相比显著升高;LASS2/TMSG-1显著下降(1.74±0.38ng/ml vs 3.02±0.49ng/ml)(P<0.05)。COX回归分析结果显示,肿瘤分化程度、肿瘤TNM分期、LASS2/TMSG-1、TAK1及TAB1表达水平是影响结肠癌患者临床预后的独立因素。LASS2/TMSG-1、TAK1、TAB1表达水平之间无显著相关性(P>0.05);患者预后与LASS2/TMSG-1、TAK1、TAB1表达水平具有显著相关性(P<0.05)。[结论] LASS2/TMSG-1、TAK1、TAB1的表达水平可能与结肠癌的发生及发展具有相关性,且与患者临床预后相关。

[Objective] To investigate the expression and clinical value of tumor metastasis suppressor gene-1(LASS2/TMSG-1),transforming growth factor activates kinase 1(TAK1) and transforming growth factor activated kinase binding protein 1(TAB1) in colon cancer. [Methods] The colon cancer tissues and normal colon tissue samples were collected from 68 patients with colon cancer. The real-time polymerase chain reaction(RT-PCR)was utilized to analyze the mRNA expression level of LASS2/TMSG-1,TAK1 and TAB1. The relationship of the expression level of LASS2/TMSG-1,TAK1,TAB1 with clinical pathological features and prognosis of patients was analyzed. [Results] The expression level of TAK1(0.76±0.15 ng/ml),TAB1(1.46±0.20 ng/ml) in colon cancer tissues was significantly higher than that in normal tissues(0.57±0.15 ng/ml,1.21±0.19 ng/ml),while the level of LASS2/TMSG-1(1.52±0.36 ng/ml) was significantly lower than that in normal tissues(2.36±0.43 ng/ml)(P<0.05).The expression level of LASS2/TMSG-1,TAK1,TAB1 was significantly correlated with tumor differentiation and TNM stage(P<0.05). There was 8 patients(11.76%) developed tumor recurrence/metastasis at 12 months of follow-up. The level of TAK1,TAB1 of patients with tumor recurrence/metastasis(0.96±0.15 ng/ml,1.68±0.19 ng/ml)were significantly higher than those without tumor recurrence/metastasis(0.52±0.13 ng/ml,1.26±0.20 ng/ml),while the level of LASS2/TMSG-1 of patients with tumor recurrence/metastasis were significantly lower than those without tumor recurrence/metastasis(1.74±0.38 ng/ml vs 3.02±0.49 ng/ml)(P<0.05). Tumor differentiation degree,TNM stages,the LASS2/TMSG-1,TAK1,TAB1 level was the independent factors of colon cancer analyze by COX Regression analysis. There was no significant correlation between LASS2/TMSG-1,TAK1 and TAB1(P>0.05). The prognosis of patients was significantly correlated with the expression levels of LASS2/TMSG-1,TAK1 and TAB1(P<0.05). [Conclusions] The expression levels of LASS2/TMSG-1,TAK1 and TAB1 may be correlated with the occurrence and development of colon cancer,as well as related to the clinical prognosis of patients.