基于网络药理学探讨湿疹溻渍方治疗湿疹的作用机制

Mechanism of Shizhen Tazi Decoction in the Treatment of Eczema:A Network-Pharmacological Study

目的:利用网络药理学方法探索湿疹溻渍方(SZTZF)治疗湿疹的物质基础与作用机制。方法:借助TCMSP数据库筛选每味药物的有效活性成分和作用靶点,基于GeneCards数据库筛选与湿疹相关的靶点,确定药物作用靶点与湿疹疾病靶点的交集基因,并进一步构建湿疹溻渍方有效成分靶点-湿疹疾病靶点的调控网络;应用STRING平台构建蛋白相互作用(PPI)网络模型;同时对二者共同靶点进行GO和KEGG通路分析。结果:共计筛选出49个有效活性成分,对应66个相关的作用靶点,其中有23个靶点与湿疹密切相关。PPI分析结果表明,白细胞介素、簇分化抗原、性激素受体及相关肿瘤蛋白在网络中占据核心地位。GO功能包括核因子受体活性、转录因子活性、类固醇激素受体活性等。KEGG共富集得到6条信号通路,主要为炎性反应、免疫调节、激素抵抗等相关信号通路。结论:湿疹溻渍方中有效活性成分可能通过作IL-6、GASP3、JUN等多个靶点进而调节多条信号通路来起到抑制湿疹炎性反应的作用,研究结果将为进一步研究提供重要信息依据。

Objective:To explore the material basis and mechanism of Shizhen Tazi Decoction(SZTZF)in the treatment of eczema using network pharmacology.Methods:The Platform of Pharmacology Technology of traditional Chinese Medicine system(TCMSP)was used in the screening of the active components and action targets of SZTZF.Correspondingly,the targets related to eczema were screened based on GeneCards database.The intersection genes between drug action targets and eczema disease targets were determined based on the analysis of the two clusters of targets,and the regulatory network of SZTZF′s effective component target-eczema disease target was further constructed.Protein interaction network(PPI)model was constructed with STRING platform.At the same time,the genetic ontology(GO)and the Kyoto encyclopedia of genes and genomes(KEGG)pathway analysis were conducted.Results:Through database analysis,66 targets related to 49 main active components of SZTZF and eczema were selected,of which 23 were closely related to eczema.The results of PPI analysis showed that interleukin,cluster differentiation antigen,sex hormone receptor and related tumor proteins occupy the core position in the network.The genetic ontology(GO)functions of TCM-disease targets included nuclear receptor activity,transcription factor activity,steroid hormone receptor activity and so on.By KEGG enrichment analysis,six signal pathways related to the main components of SZTZF were selected,which were mainly related to inflammation,immune regulation,hormone resistance,etc.Conclusion:The effective active ingredients in SZTZF may act as multiple targets such as IL-6,GASP3,and JUN and then regulate multiple signaling pathways to play a role in inhibiting the inflammatory response of eczema.The research results will provide important information for further research.