中耳胆脂瘤上皮TNF-α表达水平与听力损失的相关性研究

Correlation between the expression level of TNF-αin middle ear cholesteatoma epithelium and hearing loss

目的探讨中耳胆脂瘤上皮肿瘤坏死因子-α(TNF-α)表达水平与患者的听力损失的相关性。方法选择2017-01~2020-01新疆维吾尔自治区人民医院收治的慢性中耳炎患者89例,其中中耳胆脂瘤患者68例(观察组),慢性化脓性中耳炎活动期患者21例(对照组)。比较两组术前气导听阈、骨导听阈和骨气导差。于中耳鼓窦入口处取患者胆脂瘤上皮(观察组)或中耳病变黏膜组织(对照组),比较两组组织样本TNF-α表达水平,并分析中耳胆脂瘤上皮TNF-α表达水平与患者的听力损失的相关性。结果观察组气导听阈和骨气导差水平显著高于对照组(P<0.05);但两组骨导听阈水平比较差异无统计学意义(P>0.05)。观察组胆脂瘤上皮TNF-α表达水平为(18.64±8.55)ng/L,对照组中耳鼓窦入口病变黏膜的TNF-α表达水平为(28.29±5.64)ng/L,差异有统计学意义(P<0.05)。胆脂瘤上皮组织TNF-α水平与气导阈值、骨导阈值和骨气导差均呈正相关(P<0.05)。结论中耳胆脂瘤上皮TNF-α表达水平与患者的听力损失呈正相关。

Objective To investigate the correlation between the expression level of tumor necrosis factor-α(TNF-α)in middle ear cholesteatoma epithelium and the hearing loss of patients.Methods Eighty-nine patients with chronic otitis media admitted to the People′s Hospital of Xinjiang Uygar Autonomous Region from January 2017 to January 2020 were selected,including 68 patients with middle ear cholesteatoma(observation group)and 21 patients with active chronic suppurative otitis media(control group).The preoperative air conduction threshold,bone conduction threshold and bone air conduction difference were compared between the two groups.At the entrance of the middle ear tympanic sinus,the cholesteatoma epitheliums were taken from the observation group and the mucosal tissues of middle ear lesions were taken from the control group.The expression levels of TNF-αin tissue samples from the two groups were compared.The correlation between the expression level of TNF-αin the cholesteatoma epithelium of the middle ear and the hearing loss of the patients was analyzed.Results The levels of air conduction threshold and bone air conduction difference in the observation group were significantly higher than those in the control group(P<0.05);but there was no statistically significant difference in the level of bone conduction threshold between the two groups(P>0.05).The expression level of TNF-αin the cholesteatoma epitheliums in the observation group was(18.64±8.55)ng/L and that in the mucosal tissues of middle ear lesions at the entrance of the middle ear tympanic sinus in the control group was(28.29±5.64)ng/L,with a significant difference between the two groups(P<0.05).The level of TNF-αin cholesteatoma epithelial tissues was positively correlated with air conduction threshold,bone conduction threshold and bone air conduction difference(P<0.05).Conclusion The expression level of TNF-αin the cholesteatoma epithelium of the middle ear is positively correlated with the patients′hearing loss.