CD34、CD10、Ki-67、细胞周期蛋白D1联合应用在乳腺良性叶状肿瘤诊断中的价值

Value of combined application of CD34,CD10,Ki-67 and CyclinD-1 in the diagnosis of benign phyllodes tumors of breast

目的观察乳腺良性叶状肿瘤和纤维腺瘤的镜下形态学特点与免疫表型特征,并进一步探讨CD34、CD10、Ki-67、细胞周期蛋白D1(CyclinD-1)联合应用在两者鉴别诊断中的价值。方法筛选芜湖市第一人民医院2016-2018年乳腺良性叶状肿瘤62例,纤维腺瘤73例,观察HE组织学特征,并用免疫组化检测CD34、CD10、Ki-67、CyclinD-1在肿瘤间质中的表达情况。结果良性叶状肿瘤与纤维腺瘤中CD34的高表达(++及以上)阳性率分别为93.5%,90.4%(P>0.05);CD10的高表达(++及以上)阳性率分别为72.6%,45.2%(P<0.05);Ki-67的阳性表达率分别为27.4%,6.8%(P<0.05);CyclinD-1的阳性表达率分别为77.4%,47.9%(P<0.05);结论良性叶状肿瘤与纤维腺瘤免疫表型存在差异,CD34、CD10、Ki-67、CyclinD-1的联合应用对两者的鉴别诊断及病人的预后评估具有较大参考意义。

Objective To observe the microscopic morphological and immunophenotypic features of benign phyllodes and fibroadenomas of the breast,and to further explore the value of CD34,CD10,Ki-67 and CyclinD-1 in the differential diagnosis between them.Methods Sixty-two cases of breast benign phyllodes tumors and 73 cases of fibroadenoma in the First People’s Hospital of Wuhu City from 2016 to 2018 were screened.The histological characteristics of HE were observed.The expressions of CD34,CD10,Ki-67 and CyclinD-1 in the stroma of breast tumors were detected by immunohistochemistry.Results The positive rates of CD34(++ and above)in benign phyllodes tumors and fibroadenomas were 93.5% and 90.4%,respectively(P>0.05),the positive rates of CD10(++ and above)were 72.6% and 45.2%,respectively(P<0.05),the positive rates of Ki-67 were 27.4% and 6.8%,respectivelty(P<0.05),and the positive rates of clinD-1 were 77.4% and 47.9% respectively(P<0.05).Conclusion There are differences in immunophenotypes between benign phyllodes tumors and fibroadenomas.The combined use of CD34,CD10,Ki-67 and CyclinD-1 is of great significance in differential diagnosis and prognosis evaluation of patients with benign phyllodes tumors.