细胞特异性核酸适配体的筛选及评价策略

Screening and Evaluation Strategies of Cell-specific Nucleic Acid Aptamers

细胞特异性核酸适配体作为一个细胞表面信息的识别分子受到了广泛关注,其筛选有别于普通的小分子和大分子,在筛选过程中细胞活力的保持,死细胞干扰的去除及最终适配体运用的可行性等都较为重要。细胞适配体的筛选始终是研究的一个重点,基于细胞表面标志物、全细胞、组织和体内几个方面来揭示其筛选方式,同时将筛选出的适配体从亲和力、特异性、细胞活力、临床组织和体内可行性几个方面进行评价,以期为细胞适配体的筛选和评价方式提供一定参考。

As recognition molecules of cell surface information,cell-specific nucleic acid aptamers have received extensive attentions. The screening method is different from ordinary small molecules and macromolecules. The maintenance of cell viability,the removal of dead cell interference and the feasibility of the application of the aptamer are all important in the screening process. Screening of cell aptamers has always been a focus of research. This paper reveals the screening methods based on cell surface markers,whole-cell,tissue and in vivo. Meanwhile,the screened aptamers will be evaluated from the aspects of affinity,specificity,cell viability,and the feasibility in clinical tissue and in vivo,aiming at providing reference for the selection and evaluation of cell-specific nucleic acid aptamers.