Phaeosphaeria sp.HD-06菌株对马唐的致病性分析及初步鉴定

Pathogenicity of Phaeosphaeria sp.HD-06 against Digitaria sanguinalis and the preliminary identification of the strain

为了得到能够有效抑制马唐Digitaria sanguinalis生长的生防微生物,在农田采集马唐种子,进行萌发试验,观察种子的萌发状况,从发病的种子和马唐幼苗基部分离致病菌,通过形态学观察以及ITS序列分析对致病菌进行鉴定。结果从罹病的马唐幼苗基部分离得到一株致病菌HD-06,该菌株的发酵液用水稀释至60%浓度时,对马唐种子的萌发抑制率和幼苗发病率均达到100%,这说明菌株HD-06对马唐有较好的生物防治作用。根据其形态学特点及ITS序列分析结果,鉴定菌株HD-06为暗球腔菌属Phaeosphaeria的真菌。这些结果为深入研究其对马唐的防治作用奠定了基础。

In order to obtain the effective biocontrol microorganism for inhibiting the growth of Digitaria sanguinalis,the seeds of crabgrass were collected in the farmland,and seed germination test was carried out.The germinating situation was tracked over time;meanwhile,the pathogens were isolated from the diseased seeds and the seedling base,and morphological observation and ITS sequence analysis were conducted to identify the pathogenic fungi.The results showed that a disease-causing strain named HD-06 was successfully isolated from diseased crabgrass seedling base.When the fermentation broth of strain HD-06 was diluted by 60%with water,the germination inhibition rate and seedling disease incidence rate of crabgrass reached 100%after treatment.It indicated that strain HD-06 had a good biological control effect on crabgrass.According to the morphological characteristics and ITS sequence analysis results,strain HD-06 was identified as a fungus of the genus Phaeosphaeria.These results laid a foundation for further research on its control effect on crabgrass.