柑橘大实蝇NPC2基因的序列分析和组织表达模式

Sequence analysis and tissue expression pattern of Niemann-Pick type C2 gene in Bactrocera minax

柑橘大实蝇是柑橘上的重要害虫,主要为害柑橘果实。NPC2是一种小分子蛋白,参与了脊椎动物的脂质代谢,最近有研究表明,NPC2有着类似于昆虫气味结合蛋白的功能,参与了昆虫的化学通讯。为了明确NPC2基因在柑橘大实蝇成虫组织中的分布,本研究从柑橘大实蝇转录组中,鉴定了NPC2基因,命名为BminNPC2。通过生物信息学方法分析了该基因序列特征并构建了系统进化树,采用实时荧光定量PCR技术研究了BminNPC2基因在柑橘大实蝇成虫组织中的表达模式。结果表明:BminNPC2基因开放阅读框为456 bp,编码151个氨基酸,等电点为6.10,分子量大小为16.66 kD,含有6个半胱氨酸。BminNPC2基因在柑橘大实蝇头部(去掉触角)和腹部表达量最高。据此结果我们在文中对BminNPC2基因功能进行了简要讨论。

Bactrocera minax,a major citrus pest,mainly damages citrus fruits.NPC2 is a type of small protein involved in lipid metabolism in vertebrates.Recent studies showed that NPC2s had the similar function to odorant binding proteins and participated in chemical communication of insects.In order to clarify the distribution of NPC2 gene in adult citrus flies,the NPC2 gene was obtained from B.minax through transcriptome analysis,which was named BminNPC2.The sequence characteristics of the NPC2 gene were analyzed using bioinformatics tools and a phylogenetic tree was generated to characterize the NPC2 gene.The tissue expression pattern of NPC2 gene was determined by the real-time PCR.The results showed that the open reading frame(ORF)of BminNPC2 was 456 bp in length,encoding 151 amino acid residues,with a deduced molecular weight(MW)of 16.66 kD and an isoelectric point(pI)of 6.10.It had six cysteines.The expression level of BminNPC2 gene in the head without antennae and abdomen was high.Based on these results,we briefly discussed the functions of BminNPC2 gene.