lncRNA SCGB1B2P通过调控SPRY2基因表达对肾癌细胞增殖和侵袭的影响

Effects of lncRNA SCGB1B2P on the Proliferation and Invasion of Renal Cell Carcinoma Cells by Regulating SPRY2 Expression

目的探讨长链非编码(long non-coding RNA,lncRNA)SCGB1B2P在肾癌中的表达及影响肾癌细胞增殖和侵袭能力的作用机制。方法采用荧光实时定量PCR(qPCR)检测82例肾癌组织和肾癌细胞株中SCGB1B2P的表达水平。以SCGB1B2P表达水平最低的OS-RC-2细胞为转染对象,采用脂质体转染表达SCGB1B2P的质粒(实验组)或表达无意义序列的质粒(对照组)。采用qPCR检测各组细胞SCGB1B2P的表达水平。MTT法和Matrigel侵袭实验检测肾癌细胞的增殖能力和侵袭能力。qPCR和Western blot法分别检测SPRY2(sprouty RTK signaling antagonist 2)基因表达。结果肾癌组织中SCGB1B2P的表达水平低于癌旁组织(P<0.01),肾癌细胞中SCGB1B2P的表达水平低于正常肾小管上皮细胞(P<0.05),实验组OS-RC-2细胞中SCGB1B2P的表达水平明显高于对照组(P<0.01),上调SCGB1B2P可显著抑制肾癌细胞的增殖能力和侵袭能力(P<0.05),上调SCGB1B2P可显著促进肾癌OS-RC-2细胞中SPRY2基因的表达(P<0.01)。结论SCGB1B2P在肾癌中表达明显降低,上调SCGB1B2P可显著抑制肾癌OS-RC-2细胞的增殖和侵袭能力,其可能的作用机制是SCGB1B2P可促进SPRY2基因表达。

Objective To investigate the expression of long non-coding RNA(lncRNA)SCGB1B2P in renal cell carcinoma and its mechanism of effect on proliferation and invasion of renal cell carcinoma.Methods The expression level of SCGB1B2P in 82 renal cell carcinoma and renal cancer cell lines was detected by real-time quantitative PCR(qPCR).The OSG-RC-2 cells with the lowest expression level of SCGB1B2P were transfected with plasmids expressing SCGB1B2P(experimental group)or plasmids expressing nonsense sequences(control group)by liposome.The expression level of SCGB1B2P in each group was detected by qPCR.The proliferation and invasion ability of renal cancer cells were detected by MTT assay and Matrigel invasion assay.The expression of SPRY2 gene was detected by qPCR and Western blot,respectively.Results The expression level of SCGB1B2P in renal cell carcinoma was lower than that in adjacent tissues(P<0.01).The expression level of SCGB1B2P in renal cancer cells was lower than that in normal renal tubular epithelial cells(P<0.05).The expression level of SCGB1B2P in the OS-RC-2 cells of the experimental group was significantly higher than that of the control group(P<0.01).Up-regulation of SCGB1B2P significantly inhibited the proliferation and invasion of renal cancer cells(P<0.05).Up-regulation of SCGB1B2P significantly promoted the expression of SPRY2 gene in renal cell carcinoma OS-RC-2 cells(P<0.01).Conclusion The expression of SCGB1B2P was significantly decreased in renal cell carcinoma.Up-regulation of SCGB1B2P significantly inhibited the proliferation and invasion of renal cell carcinoma OS-RC-2 cells.The possible mechanism of action was that SCGB1B2P could promote SPRY2 gene expression.