肿瘤坏死因子诱导蛋白样因子-2、IgE对哮喘患儿病情发生发展的影响

Effect of T tumor necrosis factor-induced protein 8 like-2 and immunoglobulin E on the occurrence and development of children with asthma

目的分析肿瘤坏死因子诱导蛋白样因子-2(TIPE2)、免疫球蛋白E(IgE)对哮喘患儿病情发生发展的影响,为儿童哮喘的诊疗提供依据。方法选取2016年1月-2018年12月于郑州儿童医院进行哮喘治疗的患儿100例,同时选取98例正常健康体检儿童作为对照组。检测两组儿童TIPE2 mRNA和蛋白表达水平,检测血清中IgE水平和各个指标的准确率、敏感性情况。结果哮喘组患儿单个核细胞的TIPE2 mRNA和蛋白表达水平均明显低于对照组(t=15.165、21.265,P<0.001);血清内ⅠgE表达明显高于对照组(t=138.139,P<0.001)。TIPE2+IgE联合诊断的特异性、敏感性均显著高于TIPE2诊断与IgE诊断(χ2=14.624、13.455、18.000、14.559,P<0.001)。给予药物治疗后发现,与治疗前相比哮喘患儿体内TIPE2表达水平明显上升,而IgE表达明显下降(t=12.975、112.480,P<0.001)。结论TIPE2、IgE对哮喘患儿病情发生发展有重要影响,治疗过程中应密切关注其变化,为诊断和治疗儿童哮喘提供了新的思路。

Objective To analyze the effects of T tumor necrosis factor-induced protein 8 like-2(TIPE2)and immunoglobulin E(IgE)on the occurrence and development of asthma in children,in order to provide evidence for the diagnosis and treatment of children with asthma.Methods A total of 100 children with asthma treated in Zhengzhou Children's Hospital from January 2016 to December 2018 were enrolled in this study,and 98 children who took physical examinations at the same period were selected as control group.The expression levels of TIPE2 mRNA and protein,and the serum IgE level were tested in two groups of children,and the accuracy and sensitivity of various indicators were analyzed.Results The levels of TIPE2 mRNA and protein expression of mononuclear cells in patients with asthma were significantly lower than those in control group(t=15.165,21.265,P<0.001).The expression of IgE in asthma group was significantly higher than that in control group(t=138.139,P<0.001).The specificity and sensitivity of TIPE2 in combination with IgE for asthma diagnosis were significantly higher than those of only using TIPE2 or IgE for asthma diagnosis(χ2=14.624,13.455,18.000,14.559,P<0.001).After drug treatment,it was found that the expression level of TIPE2 in children with asthma increased significantly compared with that before treatment,while the expression of IgE decreased significantly(t=12.975,112.480,P<0.001).Conclusion s TIPE2 and IgE have important effects on the development of asthma children.The changes should be closely monitored during the treatment process,so as to provide new ideas for the diagnosis and treatment of asthma in children.