顺铂对人骨肉瘤U2OS细胞增殖、迁移和凋亡的影响

Effect of cis-platinum on proliferation,migration and apoptosis of human osteosarcoma U2OS cells

目的探讨顺铂(DDP)对人骨肉瘤U2OS细胞增殖、迁移和凋亡的影响。方法U2OS细胞用不同浓度(10、20、40μmol/L)DDP处理6、12、24、48、72 h,分别用划痕实验、CCK-8法、流式细胞术、Western blot观察细胞迁移、增殖、凋亡、凋亡相关蛋白变化。结果U2OS细胞迁移能力随DDP浓度增大、时间延长而降低。DDP作用24 h后,U2OS细胞生长活性随着DDP浓度增加而下降(P<0.01或0.05),而细胞凋亡率明显升高(P<0.01或0.05)。U2OS细胞凋亡相关蛋白cleaved-PARP、p-ERK/ERK、caspase-9、Bax表达随DDP浓度升高而增加(P<0.01),而Bcl-2表达减少(P<0.01)。结论DDP可抑制U2OS细胞增殖和迁移,并通过上调cleaved-PARP、p-ERK/ERK、caspase-9、Bax表达及下调Bcl-2表达促进细胞凋亡。

Objective To study the effect of cis-platinum(DDP)on proliferation,migration and apoptosis of human osteosarcoma U2 OS cells.Methods U2 OS cells were treated with different concentrations(10,20,40μmol/L)of DDP for 6,12,24,48,72 h.The migration,proliferation,apoptosis,and apoptosis-associated proteins of U2 OS cells were etected by scratch,CCK-8,flow cytometry,and Western blot,respectively.Results DDP suppressed migration of U2 OS cells in concentration-and time-dependent manner.After treated with DDP for 24-72 h,growth activity of U2 OS cells decreased,while apoptotic rate increased as incremental DDP concentrations(P<0.01 or 0.05).Overexpression of cleaved PARP,pERK/ERK,caspase-9 and Bax,and downexpression of Bcl-2 were observed as DDP concentrations(P<0.01).Conclusion DDP could inhibit proliferation and migration,and promote apoptosis in U2 OS cells through upregulation of cleaved PARP,pERK/ERK,caspase-9 and Bax,and downregulation of Bcl-2.