伊维菌素对宫颈癌Hela细胞增殖和凋亡的影响

Effect of ivermectin on proliferation and apoptosis of cervical cancer Hela cells

目的探讨伊维菌素对人宫颈癌Hela细胞增殖和凋亡的影响。方法0.1、1、3μmol/L伊维菌素处理Hela细胞,MTT、Edu法检测细胞活性,流式细胞术检测细胞凋亡,蛋白印迹检测p-JNK、JNK、NF-κB p65、Bcl-2、Caspase-3、Caspase-9表达。结果伊维菌素呈浓度依赖性抑制Hela细胞增殖、诱导凋亡(P<0.01),上调p-JNK、Caspase-3、Caspase-9表达,下调NF-κB p65、Bcl-2表达。结论伊维菌素可能通过上调JNK信号通路诱导Hela细胞凋亡,下调NF-κB信号通路抑制Hela细胞增殖。

Objective To investigate the effect of ivermectin on proliferation and apoptosis of cervical cancer Hela cells.Methods After treated with 0.1,1,3μmol/L ivermectin,viability,apoptosis,and expression of p-JNK,JNK,NF-κB p65,Bcl-2,Caspase-3 and Caspase-9 in Hela cells were determined by MTT,Edu,flow cytometry,Western blot,respectively.Results Ivermectin inhibited proliferation,induced apoptosis(P<0.01),up-regulated expression of p-JNK,Caspase-3 and Caspase-9,and down-regulated expression of NF-κB p65 and Bcl-2 in Hela cells in a dose-dependent manner.Conclusion Ivermectin could induce apoptosis via up-regulation of JNK pathway and inhibit proliferation through down-regulation of NF-κB signaling in Hela cells.