白术茯苓多糖复方对断奶仔猪生长性能和免疫功能的影响

Effects of Atractylodes macrocephalaon and Poria cocos Polysaccharide Compound on Growth Performance and Immune Function of Weaned Piglets

本研究旨在探讨断奶仔猪饲粮中添加白术茯苓多糖复方对其生长性能和免疫功能的影响。试验选用体重接近、健康状况良好的23日龄"杜×长×大"三元杂交断奶仔猪150头,采用单因素试验设计,将仔猪随机分为6组,抗生素组(添加硫酸黏杆菌素20 mg/kg+杆菌肽锌40 mg/kg+喹乙醇100 mg/kg)、空白组(既不添加任何抗生素也不添加白术茯苓多糖复方)和白术茯苓多糖复方添加组(分别添加0.02%、0.04%、0.06%和0.08%白术茯苓多糖复方),每组5个重复,每个重复5头猪。试验期27 d。结果表明:1)与抗生素组和空白组相比,饲粮中添加白术茯苓多糖复方显著提高了平均日增重(P<0.05),并且显著降低了料重比(P<0.05)。2)与空白组相比,抗生素组和0.06%白术茯苓多糖复方组腹泻率显著降低(P<0.05)。3)饲粮添加0.02%、0.04%、0.06%和0.08%白术茯苓多糖复方后,血清免疫球蛋白(Ig)A、IgG和IgM含量与空白组和对照组差异不显著(P>0.05);4)与抗生素组相比,饲粮添加0.06%白术茯苓多糖复方显著降低了血清肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的含量(P<0.05),且添加0.04%和0.06%白术茯苓多糖复方显著提高了血清白细胞介素-10(IL-10)的含量(P<0.05)。5)与抗生素组和空白组相比,0.06%和0.08%白术茯苓多糖复方组空肠黏膜Toll样受体4(TLR-4)、TNF-α和IL-6 mRNA相对表达量显著降低(P<0.05),而IL-10 mRNA相对表达量显著提高(P<0.05)。由此可知,白术茯苓多糖复方可以提高断奶仔猪的生长性能,缓解断奶仔猪腹泻,提高仔猪免疫水平。在本试验条件下,仔猪饲粮中白术茯苓多糖复方的适宜添加水平为0.06%。

This experiment was conducted to study the effects of Atractylodes macrocephalaon polysaccharide(AMP)and Poria cocos polysaccharide(PCP)compound on growth performance and immune function of weaned piglets.A total of 150 piglets(Duroc×Landrace×Yorkshire)weaned at 23 days of age were assigned randomly to one of six groups(five replicates per group and five pigs per replicate):antibiotic group(20 mg/kg colistin sulfate+40 mg/kg zinc bacitracin and 100 mg/kg olaquindox),blank group(without antibiotics and AMP and PCP compound),AMP+PCP compound groups(adding 0.02%,0.04%,0.06%and0.08%AMP+PCP compound,respectively).The experiment lasted for 27 days.The results showed as follows:1)compared with the antibiotic group and blank group,AMP+PCP compound significantly increased the average daily weight gain(ADG)of piglets and reduced the feed-to-weight ratio of weaned piglets(P<0.05).2)Compared with the blank group,the diarrhea rate of 0.06%AMP+PCP compound group reduced significantly(P<0.05).3)When adding 0.02%,0.04%,0.06%and 0.08%AMP+PCP compound,no significant differences were observed for serum immune globulin(Ig)A,IgG and IgM contents compared with the antibiotic group and blank group(P>0.05).4)Compared with antibiotics group,the serum tumor necrosis factor-α(TNF-α)and interleukin(IL)-6 contents significantly reduced when adding 0.06%AMP+PCP compounds in diets(P<0.05),and the serum IL-10 content significantly increased when adding 0.04%and 0.06%AMP+PCP compounds in diets(P<0.05).5)Consistently,compared with antibiotic group and control group,the relative expression levels of TLR-4,TNF-αand IL-6 mRNA significantly reduced(P<0.05),and the relative expression level of IL-10 mRNA significantly increased when adding 0.06%and 0.08%AMP+PCP compounds in diets(P<0.05).These results indicate that the AMP+PCP compound can improve the growth performance of weaned piglets,alleviate diarrhea of weaned piglets and has beneficial effects on immune function of weaned piglets.