杭白菊对肾损伤小鼠的干预效果及可能作用机制

Intervention effect of Dendranthema morifolium on mice with renal injury and its potential mechanism

目的探讨杭白菊对肾损伤小鼠的干预效果及可能作用机制。方法将50只KM小鼠随机分为对照组、模型组以及杭白菊低、中、高浓度组,每组10只。除对照组外,其余4组采用腹腔注射四氯化碳建立小鼠肾损伤模型;同时,杭白菊低、中、高浓度组分别给予150、300、600 mg/mL的杭白菊提取液灌胃,对照组、模型组给予等量生理盐水。连续给药6周后,检测5组小鼠血清白细胞介素6(IL-6)、尿素氮水平,以及血清和肾组织超氧化物歧化酶(SOD)活性、丙二醛水平,并观察各组小鼠肾组织病理学结构。结果模型组和各浓度杭白菊组小鼠的肾小体、肾小管的结构和形态出现不同程度的损坏,肾组织内有白细胞浸润,但各浓度杭白菊组以上改变均轻于模型组。模型组血清尿素氮及IL-6水平均高于对照组,而各浓度杭白菊组的血清尿素氮水平以及杭白菊高浓度组的IL-6水平均低于模型组(均P<0.05)。模型组肾组织和血清SOD活性低于对照组,而血清丙二醛水平均高于对照组(P<0.05);而各浓度杭白菊组肾组织和血清SOD活性均高于模型组,且血清丙二醛水平均低于模型组(均P<0.05);杭白菊高浓度组血清SOD活性高于杭白菊中浓度组,血清丙二醇水平低于杭白菊低浓度组(均P<0.05)。结论杭白菊对四氯化碳诱导的小鼠肾损伤具有改善作用,且高浓度(600 mg/mL)时效果最佳,其机制可能与杭白菊可减少炎症因子生成、提高机体清除自由基能力有关。

Objective To investigate the intervention effect of Dendranthema morifolium on mice with renal injury and its potential mechanism.Methods Fifty KM mice were randomly divided into control group,model group,low-,medium-and high-dose Dendranthema morifolium groups,with 10 mice in each group.Except the control group,the remaining four groups received intraperitoneal injection of carbon tetrachloride(CCl 4)to establish a mouse model of renal injury;in addition,the low-,medium-and high-dose Dendranthema morifolium groups were given 150,300,600 mg/mL of Dendranthema morifolium extracts by gavage,respectively,the control group and the model group were given equivalent amount of normal saline.After six consecutive weeks of administration,the levels of serum interleukin(IL)-6 and blood urea nitrogen(BUN),as well as serum and renal tissue superoxide dismutase(SOD)activities and malondialdehyde level were detected in the five groups,and the histopathological structure of the kidney was observed in the mice of each group.Results Mice in the model group and the Dendranthema morifolium groups exhibited various-degree damage to renal corpuscular or tubular structure and morphology,and renal tissue with neutrophil infiltration inside,however,the Dendranthema morifolium groups had milder changes mentioned above than the model group.The model group had higher serum BUN and IL-6 levels than the control group,whereas the Dendranthema morifolium groups exhibited a lower serum BUN level,and the high-dose Dendranthema morifolium group reported a lower IL-6 level as compared with the model group(all P<0.05).Compared with the control group,the model group had lower renal tissue and serum SOD activities and higher serum malondialdehyde level(P<0.05);furthermore,the Dendranthema morifolium groups obtained higher renal tissue and serum SOD activities and lower serum malondialdehyde level in comparison with the model group(all P<0.05);the high-dose Dendranthema morifolium group had a higher serum SOD activity than the medium-dose Dendranthema morifolium group,along with a lower serum malondialdehyde level than the low-dose Dendranthema morifolium group(all P<0.05).Conclusion Dendranthema morifolium has effects on improving CCl 4-induced renal injury in mice,and high-dose(600 mg/mL)achieves the optimal efficacy.The mechanism may be related to reducing the production of inflammatory factors and improving the ability to scavenging free radical of the body.