基于马尾松转录组测序的产脂相关SNPs初步分析

Preliminary Analysis of SNPs Related to Rosin Formation in Pinus massoniana Based on Transcriptome Sequencing

为明确与产脂紧密相关的SNP分子标记在马尾松(Pinus massoniana)育种中的适用性,加快产脂性状遗传改良,基于马尾松高产脂无性系与普通产脂无性系3个组织(顶芽、针叶和韧皮部)的转录对数据进行基因表达分析,并开发ESTSNP标记,为产脂性状的分子标记辅助选择提供适合标记。通过对2 329个差异表达序列进行筛选,在374条Unigene中检索到2 192个SNP位点。韧皮部独有、针叶与韧皮部共有及3个组织共有的Unigene 125条。转换和颠换分别占61.59%和38.41%。通过GO分类,参与生物过程、细胞成分和分子功能的Unigene分别为93、49和92条;在KEGG注释中,新陈代谢通路的Unigene最多,有21条。数据显示GO分类和KEGG通路的注释结果一致,相关基因主要参与代谢过程。进一步开发SNP标记,可为马尾松产脂性状候选基因关联分析提供理想工具。

In order to clarify applicability of SNP molecular markers which are closely linked to rosin formation in breeding of Pinus massoniana and to accelerate genetic improvement of rosin-formation traits, gene expression analysis was performed based on transcriptome data of three tissues(apical buds, needles and phloem) of high rosin-formation clones and common rosin-formation clones of P. massoniana. EST-SNP marker provided suitable marker for molecular marker-assisted selection of rosin-formation traits. By screening 2 329 differentially expressed sequences, 2 192 SNP loci were retrieved from 374 Unigenes. 125 Unigenes were selected,which were unique to phloem, shared by needles and phloem, and shared by three tissues. Conversion and transversion accounted for 61.59% and 38.41%, respectively. According to GO classification, there were 93, 49 and92 Unigenes involved in biological processes, cell components, and molecular functions;there were up to 21 Unigenes in metabolic pathway in KEGG annotation. These data showed that GO classification and KEGG pathway annotation results were consistent, and related genes were mainly involved in metabolic process. SNP markers could be further developed to provide ideal tool for association analysis of candidate genes for rosin-formation traits of P. massoniana.