马铃薯StSnRK1基因过表达提高烟草耐盐性研究

Study of Overexpression of StSnRK1 Gene Increases Salt Tolerance in Tobacco

为研究马铃薯蔗糖非发酵-1-型相关蛋白激酶-1基因StSnRK1对于调控植物耐盐性的促进作用,以过表达StSnRK1的烟草株系及野生型为试验材料,研究盐胁迫下StSnRK1对植株生长的影响。耐盐性鉴定结果表明,过表达StSnRK1基因显著提高烟草植株的耐盐性。实时荧光定量PCR(RT-qPCR)分析显示,StSnRK1基因显著上调脯氨酸生物合成相关基因(吡咯琳-5-羧酸合酶NtP5CS)、胚胎发育后期丰富蛋白基因(NtLEA5)和活性氧清除系统相关基因(超氧化物歧化酶NtSOD和过氧化物酶NtPOD)。同时,转基因烟草植株的SOD活性、POD活性和脯氨酸含量显著高于野生型烟草植株,丙二醛(MDA)含量和过氧化氢(H2O2)含量显著低于野生型植株。由此可见,StSnRK1基因在改良植物耐盐性方面具有重要作用,为耐盐马铃薯生物技术育种提供了理论基础。

To study the enhanced effect of potato sucrose non-fermenting-1-related protein kinase 1 gene(StSnRK1)on regulating plant salt tolerance,StSnRK1-overexpressing tobacco and wild-type(WT)tobacco plants were used to study the effect of StSnRK1 on the growth of tobacco plants under salt stress.Salt tolerance identification indicated that overexpression of StSnRK1 significantly improved salt tolerance of tobacco plants.Real-time quantitative PCR(qRTPCR)analysis indicated that StSnRK1 gene significantly up-regulated proline biosynthesis related gene(pyrroline-5-carboxylate synthase NtP5CS),late embryogenesis abundant protein gene(NtLEA5)and reactive oxygen scavenging(ROS)system related genes(superoxide dismutase NtSOD and peroxidase NtPOD)under salt stress.Meanwhile,the activities of SOD and POD and the content of proline in transgenic tobacco were significantly higher than that in wildtype tobacco plants,while the content of malondialdehyde(MDA)and hydrogen peroxide(H2O2)was significantly lower than that in wild-type tobacco plants.These results indicate that StSnRK1 gene plays an important role in improving salt tolerance of plants,providing a basis for salt-tolerant biotechnology breeding of potato.