bFGF及PDGF联合应用对大鼠胫骨骨折愈合的影响

Effects of the combined application of bFGF and PDGF on tibia fracture healing in rats

目的研究局部联合应用外源性碱性成纤维细胞生长因子(bFGF)、血小板衍生生长因子(PDGF)对大鼠胫骨骨折愈合过程的影响作用。方法清洁级成熟雄性SD大鼠48只,随机分成4组,建模。各组分别在不同时相按不同方案给药。术后2周及4周随机抽选6只大鼠取血并处死,行大体观察、X线照片、留取标本、组织学观察。测定血清中ALP、VEGF、BMP-2及IGF-1的含量。结果(1)各时间点,治疗组在骨痂大小、硬度、影像学评分及骨小梁成骨指数方面优于对照组,且联合应用最优(P<0.05)。(2)通过ALP、VEGF、BMP-2及IGF-1的检测,联合组均高于其他三组。结论大鼠胫骨骨折部位局部应用bFGF或PDGF可以促进骨折愈合,且联合应用效果优于单独应用。此过程中两因子对VEGF、BMP-2及IGF-1同向促进作用或许是其作用机制之一。

Objective To explore the joint action of basic fibroblast growth factor(bFGF)and platelet-derived growth factor(PDGF)on the healing of tibia fractures in rats.Methods Forty-eight clean grade mature male SD rats were randomized in four groups,and a right middle tibia fracture was induced in each rat.Each group received bFGF and PDGF at different timepoints.Two to four weeks after the fractures were induced,six randomly selected rats from each group were sacrificed,and blood samples were taken from the femoral artery.Gross observations and histological observations were performed,and radiographs and additional specimens were taken.The levels of ALP,VEGF,BMP-2,and IGF-1 were measured at different observation points.Results Each period after operationt,analysis of the gross specimens showed that the size,density,imaging score,and trabecular bone index of the new bone tissue formed in groups A,B,and group C were significantly higher than in group D.The group that was treated with both growth factors exhibited higher levels of ALP,VEGF,BMP-2,and IGF-1 expression than the other three groups.Conclusions Local injection of exogenous bFGF or PDGF can accelerate fracture repair,and the effect of combined application is better than that of single application.The elevated expression of VEGF,BMP-2,and IGF-1 promoted by the two factors may represent one of the mechanisms involved in this process.