穴位埋线对原发性痛经大鼠子宫组织前列腺素相关因子和核转录因子κB的影响

Effect of acupoint catgut embedding on levels of PG-related factors and NF-κB proteins in ute-rine tissues of rats with primary dysmenorrhea

目的:观察穴位埋线对原发性痛经(PD)大鼠子宫组织中前列腺素F2α(PGF2α)、环氧化酶-2(COX-2)和核转录因子κB(NF-κB)的影响,探讨穴位埋线治疗PD的可能机制。方法:SD雌性大鼠随机分为正常组、模型组、穴位埋线组、西药组,每组10只。采用苯甲酸雌二醇联合缩宫素建立PD大鼠模型。穴位埋线组大鼠于造模第1天及造模第5天予"关元""三阴交"埋线治疗;西药组大鼠予125 mg/100 mL布洛芬灌胃(0.8 mL/只)治疗,连续给药10 d。第11天,各组大鼠腹腔注射缩宫素(2 U/只)后,ELISA法检测子宫组织中PGF2α含量;Western blot法检测子宫组织中COX-2、NF-κB p65、磷酸化NF-κB(phospho-NF-κB p65)的蛋白表达水平。结果:与正常组比较,模型组大鼠子宫组织PGF2α含量及COX-2、NF-κB p65、phospho-NF-κB p65蛋白表达水平明显升高(P<0.05);与模型组比较,穴位埋线组、西药组大鼠子宫组织PGF2α含量和COX-2蛋白表达水平明显降低(P<0.05),穴位埋线组大鼠子宫组织phospho-NF-κB p65蛋白表达水平明显降低(P<0.05);与西药组比较,穴位埋线组大鼠子宫组织phospho-NF-κB p65蛋白表达水平明显降低(P<0.05)。结论:穴位埋线干预PD大鼠的作用机制可能与抑制其子宫组织中NF-κB活化,降低COX-2水平,从而有效调节PGF2α水平有关。

Objective To observe the effect of acupoint catgut embedding on levels of prostaglandin F2α(PGF2α),cyclooxygenase-2(COX-2)and nuclear factor kappa B(NF-κB)in the uteruses of rats with primary dysmenorrhea(PD),so as to explore its mechanisms underlying improvement of PD.Methods Forty female SD rats were randomly divided into normal,model,acupoint catgut embedment and medication groups,with 10 rats in each group.The PD model was established by subcutaneous injection of estradiol benzoate(0.5 mg on the 1st day,and 0.2 mg thereafter)once daily for 10 days,followed by i.p.of oxytocin 2 U(0.5 mg·5 U-1·mL-1)on the 11th day.Catgut embedment was applied to"Guanyuan"(CV4)and"Sanyinjiao"(SP6)on day 1 and 5 while modeling,and rats of the medication group received gavage of ibuprofen(1.25 mg/mL,0.8 mL/rat)once daily for 10 d.The level of PGF2αin the uterus tissuewas assayed by ELISA,and the expression levels of uterine COX-2,phospho(p)-NF-κB p65,NF-κB p65 proteins were detected by Western blot.Results Compared with the normal group,the levels of PGF2α,COX-2,p-NF-κB p65 and NF-κB p65 in the uterine tissues were significantly increased in the model group(P<0.05).Compared with the model group,the levels of PGF2αand COX-2 in both catgut embedment and medication groups as well as p-NF-κB p65 in the catgut embedment group were significantly decreased(P<0.05).Compared with the medication group,the level of p-NF-κB 65 in the catgut embedment group was significantly decreased(P<0.05).Conclusion The analgesic effect of acupoint catgut embedding may be related to its effects in down-regulating the expression of p-NF-κB and the levels of COX-2 and PGF2αin uteruses of PD rats.