上皮性卵巢癌组织中miRNA-21a/b表达及其临床意义

Expression of miRNA-21a/b in Patients with Epithelial Ovarian Cancer Tissues and Its Clinical Significance

目的探讨上皮性卵巢癌(epithelial ovarian cancer,EOC)组织中miRNA-21a/b的表达及其临床意义。方法选择卵巢癌病理组织标本88例,其中EOC组织标本68例(EOC组),良性卵巢上皮性肿瘤组织标本20例(良性组)。取所有患者手术切除的病灶组织样本,采用q PCR法检测miRNA-21a/b表达水平,调查所有患者的临床病理资料并进行相关性分析。结果EOC组的miRNA-21a、miRNA-21b相对表达水平都显著高于良性组(P<0.05)。随着临床分期的增加、组织学分化的降低以及淋巴结转移,EOC患者的miRNA-21a/b相对表达水平显著增加(P<0.05)。在EOC患者中,直线相关分析显示患者的miRNA-21a/b相对表达水平与临床分期、组织学分化、淋巴结转移成显著正相关性(P<0.05)。COX回归分析显示临床分期、组织学分化、淋巴结转移都为影响EOC组织miRNA-21a/b相对表达水平的主要因素(P<0.05)。结论EOC患者组织miRNA-21a/b呈现高表达状态,与患者的临床分期、组织学分化、淋巴结转移显著相关。

Objective To investigate the expression of miRNA-21 a/b in patients with epithelial ovarian cancer(EOC tissues and its clinical significance.Methods 88 patients with pathological tissue specimens from obstetrics and gynecology were selected that included patients of 68 EOC tissue specimens(EOC group)and 20 patients of benign ovarian epithelial tumor tissue specimens(benign group).The tissue samples of all the patients were removed,and the expression level of miRNA-21 a/b were detected by q PCR.The clinical and pathological data of all patients were investigated and given correlation analysis.Results The relative expression levels of miRNA-21 a and miRNA-21 b in the EOC group were significantly higher than those in the benign group(P<0.05).The relative expression levels of miRNA-21 a/b in the EOC patients were increased significantly with increased clinical stage,decreased histological differentiation,and lymph node metastasis(P<0.05).In the EOC patients,linear correlation analysis showed that patients’relative expression levels of miRNA-21 a/b was significantly positively correlated with clinical stage,histological differentiation,and lymph node metastasis(P<0.05).COX regression analysis showed that clinical stage,histological differentiation,and lymph node metastasis were the main factors affected the relative expression levels of miRNA-21 a/b in the EOC tissues(P<0.05).Conclusion The expression of miRNA-21 a/b in tissues of EOC patients is highly correlated with clinical staging,histological differentiation and lymph node metastasis.