桃乙烯应答因子PpERF1a的克隆与功能分析

Cloning and Functional Analysis of ERF Transcription Factor Gene PpERF1a in Peach

以‘丰光’油桃嫩梢组织为试材,克隆了1个ERF家族基因并命名为PpERF1a(ppa018178m)。该基因全长为600 bp,编码199个氨基酸,含有1个典型的AP2结构域。亚细胞定位结果显示PpERF1a定位于细胞膜和细胞核。在拟南芥中超量表达PpERF1a,共获得15个阳性转基因株系。与对照相比,转基因植株出现发育不良的表型,其中6株持续长出莲座叶,但不抽薹;6株能够抽薹开花,但不结实且生长势明显弱于对照;3株能够开花结实,但种子产量极低。这些结果表明PpERF1a在转基因拟南芥中具有调控生长发育的功能,为后期在桃中开展PpERF1a的功能研究提供了有益的启示。

In this study,an ethylene response factor was cloned from‘Fengguang’nectarine and named as PpERF1a(ppa018178m).The full length of PpERF1a was 600 bp,which encoded 199 amino acids.PpERF1a contained a single AP2 domain,which was consistent with the ERF families’features.Subcellular localization assays showed that PpERF1a was targeted to nucleus and cell membrane.Fifteen transgenic Arabidopsis plants overexpressing PpERF1a were generated.Comparing with the wild type Arabidopsis plants,transgenic plants showed obvious stunted phenotype.Six transgenic plants kept on producing rosette leaves without bolting or producing seeds.Six transgenic plants bolted and flowered,but no seed.Three transgenic plants could flower and produce seed,but the seed yield of the transgenic plants was obviously lower than that of the wild type.These results showed that PpERF1a gene regulated the growth and development of transgenic Arabidopsis plants.This study provides a technical basis for further analysis the functions of PpERF1a gene in peach growth and development.