麦麸阿拉伯木聚糖-牛血清蛋白质酶促共聚物制备及表征

Preparation and Characterization of Wheat Bran Arabinoxylan-bovine Serum Albumin Enzymatic Copolymer

研究麦麸阿拉伯木聚糖与牛血清白蛋白在漆酶的催化下形成酶促共聚物。通过用紫外分光光度法(UVvis)、十二烷基硫酸钠聚丙烯胺凝胶电泳法(SDS-PAGE)及红外光谱法(FT-IR)对酶促共聚物进行定性表征,并测定酶促共聚物与麦麸阿拉伯木聚糖及牛血清白蛋白乳化特性。结果表明,麦麸阿拉伯木聚糖在漆酶的作用下与牛血清白蛋白发生接枝反应,制得的麦麸阿拉伯木聚糖-牛血清白蛋白酶促共聚物(155.8 m^2/g,23.7 min)运用在乳状液体系中时,乳化活性及乳化稳定性优于麦麸阿拉伯木聚糖/牛血清白蛋白混合物(144.8 m^2/g,18.5 min)及麦麸阿拉伯木聚糖(75.3 m^2/g,17.5 min)及牛血清白蛋白单体(128.0 m^2/g,15.7 min)。

The enzymatic copolymers of wheat bran arabinoxylan(WBAX)and bovine serum albumin(BSA)catalyzed by laccase were studied.The enzymatic copolymer was characterized by ultraviolet spectrophotometry(UV-vis),sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)and infrared spectroscopy(FT-IR).Results showed that WBAX was grafted with BSA under the action of laccase.The emulsification activity and stability of the prepared conjugates(155.8 m^2/g,23.7 min)were better than that of the wheat bran arabinoxylan-bovine serum albumin complex(144.8 m^2/g,18.5 min)and the wheat bran arabinoxylan(75.3 m^2/g,17.5 min)or bovine serum albumin monomer(128.0 m^2/g,15.7 min).