分离紫薯果胶多糖树脂的筛选及其工艺优化

Resin Screening for the Pectic Polysaccharide of Purple Sweet Potato and Its Process Optimization

以紫薯为原材料,采用超声波辅助法提取紫薯果胶多糖,大孔吸附树脂纯化,以吸附率、解吸率和多糖保留率为评价指标,从7种大孔树脂中筛选出D296R为较优树脂,在单因素试验基础上,采用L9(34)正交设计优化。结果表明,上样液质量浓度2.0 mg/mL,上样液pH 2.0进行吸附,吸附饱和平衡后,用70%乙醇溶液洗脱,洗脱流速2.0 mL/min,洗脱液体积5 BV为较佳工艺。在此条件下紫薯果胶多糖纯度从20.7%提高到61.5%,表明大孔树脂D296R对紫薯果胶多糖具有较好的纯化效果。

With purple sweet potato as raw material,the ultrasonic-assisted extraction technology was used to study the extraction of pectin polysaccharides.The adsorption mass and desorption rate were selected as the evaluation indexes,D296R was screened from seven resins.The orthogonal experiment design was used to optimize the extraction conditions.The optimal purification technology was as follows:sample concentration of 2.0 mg/mL,sample pH 2.0,eluent flow rate of 2.0 mL/min,the volume of eluent for 5 BV.Under this condition,the purity of pectic polysaccharide was increased from 20.7%to 61.5%,which indicated that macroporous resin D296R had better purification effect on pectic polysaccharide from purple sweet potato.