不同采收时期蜂王浆抗氧化活性研究

Antioxidant Activity of Royal Jelly in Different Collection Times

为研究不同采收时间对蜂王浆品质的影响,以4-9月份采收的蜂王浆为研究对象,通过比较不同采收时间蜂王浆中蜂王浆主蛋白(MRJPs)、总水溶性蛋白和多酚的含量,分析了MRJPs与自由基清除能力和总抗氧化能力的相关性。结果表明,不同采收期蜂王浆中MRJPs含量存在显著差异(P<0.05),且6月份采收的MRJPs含量最低。水溶性蛋白及总酚含量差异不大(P>0.05)。蜂王浆自由基清除能力与MRJP1和MRJP3含量的相关系数达0.828和0.847;总抗氧化能力与MRJP1和MRJP3含量的相关系数分别为0.680和0.743。蜂王浆的抗氧化活性与其MRJPs存在一定程度的正相关,但与多酚含量相关性不明显,说明其自由基清除能力与总抗氧化活性可能是多种抗氧化性活性物质共同作用的结果。本研究为蜂王浆的抗氧化活性研究提供了参考。

To clarify the influence of different harvesting time on the quality of royal jelly, royal jelly collected from April to September were used to compare content of major royal jelly proteins(MRJPs), total soluble protein and polyphenol and to analyze the relevant between antioxidant activity and MRJPs. The results showed that there were significant differences among MRJPs(P<0.05), and the content of royal jelly harvested in June was the lowest. Content of water soluble protein and total phenol exhibited no significant differences(P>0.05), and the correlation coefficients of free radical scavenging ability and MRJP1, MRJP3 were 0.828 and 0.847 respectively;the correlation coefficients of total antioxidant capacity and MRJP1, MRJP3 were 0.680 and 0.743 respectively. There was positive correlation between the antioxidant activity of royal jelly and its major royal jelly proteins, but no significant correlation was observed between the antioxidant and content of polyphenols. This indicates the free radical scavenging ability and total antioxidant capacity of royal jelly exhibited by several antioxidants. The research provides references for the study of antioxidant activity of royal jelly.