湖南预防新冠肺炎1号方对人体细胞免疫和体液免疫的影响

The Effect of Hunan Preventing Corona Virus Disease 2019(COVID-19)No.1 Prescription(湖南预防新冠肺炎1号方)on Human Cellular Immunity and Humoral Immunity

目的:探讨湖南预防新冠肺炎1号方(预防1号方)对人体细胞免疫和体液免疫的影响。方法:选择在本院体检中心体检的健康人群398人,18~59岁为中青年组参与试验一,60岁以上为老年组参与试验二;两组试验均将受试者分为干预组和非干预组,干预组口服预防1号方4 d,非干预组未服药,检测用药前后外周血清CD4^+/CD8^+、IgA、IgM、IgG、C3、C4、IL-2、TNF及IFN-α的变化。结果:(1)试验一:干预组用药后IgA、IgM、IgG显著下降(P<0.05),而非干预组无明显变化;干预组及非干预组C3、C4均显著下降(P<0.05);干预组用药后CD4^+/CD8^+无明显变化,而非干预组CD4^+/CD8^+显著上升(P<0.05);非干预组和干预组IL-2均显著升高(P<0.05);两组用药后上述指标比较,差异无统计学意义(P>0.05)。(2)试验二:非干预组和干预组用药后IgA、IgM、IgG、C3、C4、CD4^+/CD8^+均显著下降(P<0.05)。干预组用药后IL-2显著上升(P<0.05),IFN-α显著下降(P<0.05);非干预组用药后TNF显著上升(P<0.05)。两组用药后上述指标比较,差异无统计学意义(P>0.05)。结论:湖南预防新冠肺炎1号方对年轻健康群体及老年健康人群的免疫功能有一定的调节作用,其中对年轻健康人群体液免疫的影响大于细胞免疫。

Objective:To explore the effect of Hunan Preventing Corona Virus Disease 2019(COVID-19)No.1 Prescription(Preventing No.1 Prescription)on human cellular immunity and humoral immunity.Methods:A total of 396 healthy people were selected from the physical examination center in our hospital.18-59 years old is the young and middle-aged group to participate in Experiment 1,60 years old and above is the elderly group to participate in Experiment 2.Both groups divided the subjects into intervention group and non-intervention group.The intervention group was treated with Preventing No.1 Prescription for 4 days,while the non-intervention group was not treated with the medicine.The changes of peripheral serum CD4^+/CD8^+,IgA,IgM,IgG,C3,C4,IL-2,TNF and IFN-αbefore and after medication were detected in both groups.Results:(1)Experiment 1:IgA,IgM and IgG in the intervention group decreased significantly(P<0.05),while there was no significant change in the non-intervention group.C3 and C4 in the intervention group and the non-intervention group decreased significantly(P<0.05).There was no significant change in CD4^+/CD8^+in the intervention group,while the non-intervention group CD4^+/CD8^+increased significantly(P<0.05).IL-2 was significantly increased in non-intervention group and intervention group(P<0.05).There was no statistically significant difference in the above indexes between the two groups after medication(P>0.05).Experiment 2:After treatment,the IgA,IgM,IgG,C3,C4,and CD4/CD8 of the two groups were significantly decreased(P<0.05).IL-2 increased significantly in the intervention group(P<0.05),and IFN-αdecreased significantly(P<0.05).TNF in the non-intervention group increased significantly after medication(P<0.05).There was no statistically significant difference in the above indexes between the two groups after medication(P>0.05).Conclusion:Preventing Corona Virus Disease 2019(COVID-19)No.1 Prescription has a certain regulatory effect on the immune function of young healthy people and elderly healthy people.Among them,the effect on humoral immunity of young healthy people is greater than that of cellular immunity.