妇可靖胶囊对慢性盆腔炎大鼠炎症细胞因子、氧自由基水平的影响及相关机制研究

Effect of Fukejing Capsule(妇可靖胶囊)on Inflammatory Cytokines and Oxygen Free Radicals in Rats with Chronic Pelvic Inflammation and Related Mechanisms

目的:探究妇可靖胶囊对慢性盆腔炎大鼠相关炎性细胞因子表达、氧自由基水平的影响及作用机制。方法:雌性SD大鼠随机分为空白对照组、模型组、妇乐片组和妇可靖高、中、低剂量组,除空白对照组外,其余5组大鼠采用苯酚胶浆灼烧法建立慢性盆腔炎模型。造模成功后,妇乐片组和妇可靖高、中、低剂量组分别灌胃相应药物,空白对照组及模型组分别给予等量的生理盐水,1次/d,连续给药28 d。观察各组大鼠的子宫状态及病理切片;采用酶联免疫法(ELISA)检测大鼠血清肿瘤坏死因子α(TNF-α)、抗子宫内膜抗体(EMAb)的表达情况;采用试剂盒检测大鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)的表达水平。结果:与空白对照组比较,模型组大鼠子宫组织呈现水肿、充血、粘连的病理状态,子宫重量系数升高,TNF-α、EMAb、MDA表达升高,SOD降低,差异均有统计学意义(P<0.05或P<0.01)。给药后,与模型组比较,妇可靖各剂量组子宫组织状况改善,TNF-α、EMAb、MDA表达降低,SOD表达升高,其中以中剂量组改善程度最为明显,各项指标均具有统计学意义(P<0.05或P<0.01)。妇可靖各剂量组大鼠子宫重量系数与妇乐片组比较,差异无统计学意义(P>0.05);妇可靖高剂量组大鼠TNF-α、MDA表达高于妇乐片组,SOD水平低于妇乐片组(P<0.05或P<0.01),但EMAb与妇乐片组比较,差异无统计学意义(P>0.05);妇可靖中、低剂量组大鼠EMAb低于妇乐片组(P<0.01),但TNF-α、SOD、MDA与妇乐片组比较,差异无统计学意义(P>0.05)。结论:妇可靖胶囊具有治疗慢性盆腔炎的作用,治疗效果与给药剂量具有相关性,其作用机理与调节炎症细胞因子TNF-α的表达以及改善氧自由基水平,调节EMAb抗体的水平有关。

Objective:To investigate the effect and mechanism of Fukejing capsule on the expression of related inflammatory cytokines and oxygen free radical levels in rats with chronic pelvic inflammatory disease.Methods:SD rats were randomly divided into blank control group,model group,Fule tablet group and Fukejing high,medium and low dose groups.Except the blank control group,the other 5 groups of rats were burned with phenol glue to establish a model of chronic pelvic inflammatory disease.After successful modeling,the Fule tablet group and the Fukejing high,medium and low dose groups were given the corresponding drugs by gavage,and the blank control group and the model group were given the same amount of normal saline,once a day for 28 days.Observe the uterine status and pathological sections of rats in each group.Enzyme linked immunoassay(ELISA)was used to detect the expression of rat serum tumor necrosis factor alpha(TNF-α)and anti-endometrial antibody(EMAb).The expression levels of superoxide dismutase(SOD)and malondialdehyde(MDA)in rat serum were detected by using a kit.Results:Compared with the blank control group,the uterine tissue of the model group showed pathological states of edema,hyperemia and adhesion.The uterine weight coefficient and TNF-α,EMAb,MDA expression in the model group were higher than that in the blank control group,and SOD was lower than that in the blank control group(P<0.05 or P<0.01).After administration,compared with the model group,the uterine tissue condition of each dose of fukejing group was improved,the expression of TNF-α,EMAb,MDA was decreased,and the expression of SOD was increased,especially in the medium dose group(P<0.05 or P<0.01).There was no statistically significant difference between the uterine weight coefficient of Fukejing group and Fule tablet group(P>0.05).The expression of TNF-αand MDA in Fukejing high-dose group was higher than that in Fule tablet group,the SOD level was lower than that in Fule tablet group(P<0.05 or P<0.01),but there was no statistically significant difference between the EMAb in Fukejing high-dose group and the Fule tablet group(P>0.05).The EMAb of Fukejing medium and low dose group rats was lower than that of Fule tablet group(P<0.01),but there was no statistically significant difference between TNF-α,SOD and MDA in Fukejing medium and low dose groups and Fule tablet group(P>0.05).Conclusion:Fukejing Capsule has the effect of treating chronic pelvic inflammatory disease.The therapeutic effect is related to the dosage.The mechanism of action is related to regulating the expression of inflammatory cytokine TNF-α,improving the level of oxygen free radicals and regulating the level of EMAb antibody.