一例冬季疑似牛焦虫病的检测

Detection of a Suspected Bovine Scorch worm Disease in Winter

为了检测某病牛是否感染了焦虫。试验采用无菌采取患病牛抗凝血制备血涂片,进行瑞氏染色,显微镜镜检,选取疑似存在虫体的牛血提取核酸,使用焦虫特异性引物进行PCR扩增,阳性产物测序结果提交NCBI进行比对。血涂片染色后可观察到虫体,然后使用PCR检测方法可成功扩增特异性目的条带,测序结果进行BLAST比对该基因与XM_947905.1的同源性为91.63%,结果表明该牛患有焦虫病。说明在冬季牛也可以患焦虫病。

To detect whether a sick cow is infected with scorch worms.Aseptic bovine anticoagulation was used to prepare blood smears,red staining,microscopic examination,bovine blood was selected to extract nucleic acid,PCR amplification using specific primers of scorch worm,and the sequencing results of positive products were submitted to NCBI for comparison.After staining with blood smear,the parasite could be observed and then the specific target band could be successfully amplified by PCR.The homology between the gene and XM947905.1 by BLAST was 91.63%.The results showed that the cow had scorch worm disease.It shows that in winter cattle can also suffer from scorch worm disease.