骨宝丸对维甲酸诱导小鼠骨质疏松的防治作用及机制探讨

The protective effect and mechanism of Gubao Wan(GBW)on tretinoin-induced osteoporosis in mice

目的研究骨宝丸对维甲酸诱导小鼠骨质疏松的防治作用,并探讨相关作用机制。方法将雌性BALB/c小鼠分为正常组(Control)、模型组(Model)以及骨宝丸高剂量组(GBW-H)、中剂量组(GBW-M)和低剂量组(GBW-L)。实验第1~30天,模型组和骨宝丸各剂量组小鼠灌服维甲酸(100 mg/kg)建立骨质疏松模型,同时骨宝丸各剂量组小鼠灌服药物溶液进行防治。实验结束后,取小鼠股骨,称取质量、测量股骨直径和股骨长度;利用HE染色法在光学显微镜下观察股骨组织变化;利用Micro-CT检测骨密度;采用ELISA试剂盒检测血清中BMP-2、OC、BALP、P1NP分泌水平。结果骨宝丸对维甲酸导致的小鼠股骨的质量、直径、骨密度和骨髓组织减少有防治作用,而且骨宝丸对维甲酸诱导小鼠血清OC和P1NP分泌增加、BALP和BMP-2分泌减少均有拮抗作用。结论骨宝丸可防治维甲酸诱导的小鼠骨质疏松,其作用机制可能与保护BMP-2分泌进而促进骨形成有关。

Objective To investigate the protective effect and mechanism of Gubao Wan(GBW)on tretinoin-induced osteoporosis in mice.Methods Female BALB/c mice were randomly divided into control group,model group,and GBW high dose group(GBW-H),medium dose group(GBW-M),and low dose group(GBW-L).On the days 1-30,mice in model group and GBW treated groups were treated orally with tretinoin(100 mg/kg)to establish osteoporosis model.At the same time,mice in GBW treated groups were administrated orally with GBW solution.At the end of the experiment,the femurs of the mice were collected to measure the weight,diameter,and length.The changes of femur tissue were observed with HE staining under an optical microscope.Bone mineral density(BMD)of the femurs was measured with micro-CT.The levels of BMP-2,OC,BALP,and P1NP in the serum were detected using ELISA.Results GBW significantly prevented the decrease of weight,diameter,BMD,and bone marrow tissue of the femurs in mice induced by tretinoin.Furthermore,GBW inhibited the secretion of OC and P1NP,and promoted the secretion of BMP-2 and BALP in the serum of tretinoin-induced mice.Conclusion GBW prevents from tretinoin-induced osteoporosis in mice,which may be through the protection of BMP-2 secretion and the stimulation of bone formation.