生物信息学分析筛选胆管癌新候选基因及其意义

Novel candidate genes in cholangiocarcinoma determined by intergrated bioinformatic analysis

目的应用综合生物信息学分析、筛选胆管癌新候选基因,并探讨其在预测患者预后方面的价值。方法从The Cancer Genome Atlas(TCGA)数据库下载数据,通过与癌旁组织进行比较,筛选出差异表达基因,并通过生物信息学方法对其进行深入分析。结果共筛选出差异表达的信使RNA(messenger RNA,mRNA)3538个、长链非编码RNA(long noncoding RNA,lncRNA)1434个和微RNA(microRNA,miRNA)68个。建立一个包含127个lncRNA、15个miRNA和64个mRNA的竞争性内源RNA(competing endogenous RNA,ceRNA)网络。生存分析发现,COL18A1-AS1、LINC00261、NEXN-AS1、SCRN1和SLC6A1-AS1基因对胆管癌预后具有预测价值,上述基因低表达患者的生存率均显著高于高表达患者。结论COL18A1-AS1、LINC00261、NEXN-AS1、SCRN1和SLC6A1-AS1基因可作为评估胆管癌预后的标志物,可为胆管癌的治疗提供新的潜在靶点。

Objective The novel candidate genes of cholangiocarcinoma were screened by comprehensive bioinformatics analysis,and their prognostic value was evaluated.Method The data were downloaded from the Cancer Genome Atlas(TCGA)database,and the differentially expressed genes were screened out by comparing with paracancerous specimens,and further analyzed by bioinformatics.Result A total of 3538 messenger RNAs(mRNAs),1434 long noncoding RNAs(lncRNAs)and 68 microRNAs(miRNAs)were screened for differential expression in cholangiocarcinoma tissues by comparing with paracancerous specimens.A competing endogenous RNA(ceRNA)network involving 127 lncRNAs,15 miRNAs,and 64 mRNAs was set up.In survival analysis,COL18A1-AS1,LINC00261,NEXN-AS1,SCRN1 and SLC6A1-AS1 had prognostic value on cholangiocarcinoma,and the survival rate of low-expression patients were significantly higher than high-expression patients.Conclusion COL18A1-AS1,LINC00261,NEXNAS1,SCRN1 and SLC6A1-AS1 can be used as a prognostic biomarker in cholangiocarcinoma,and provide the novel and potential therapeutic target for cholangiocarcinoma.