热休克蛋白70、镁依赖性蛋白磷酸酶1D和肿瘤坏死因子-α在甲状腺乳头状癌中的表达及其与临床病理特征的关系

Expression of heat shock protein 70,protein phosphatase magnesium-dependent 1 delta and tumor necrosis factor-αin papillary carcinoma of thyroid and their relationship with clinicopathological features

目的探讨热休克蛋白70(heat shock protein 70,HSP70)、镁依赖性蛋白磷酸酶1D(protein phosphatase magn e sium-dependent 1 delta,PPM1D)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)在甲状腺乳头状癌中的表达及与临床病理特征的关系。方法选取2016年4月至2019年3月深圳市人民医院来源的甲状腺组织病理标本92份,其中甲状腺乳头状癌组织45份、甲状腺滤泡性腺瘤组织32份、正常甲状腺组织15份。采用免疫组织化学法检测三组标本组织中HSP70、PPM1D、TNF-α表达情况,分析HSP70、PPM1D、TNF-α与临床病理特征的关系。结果甲状腺乳头状癌组织中HSP70、PPM1D、TNF-α阳性表达率均显著高于甲状腺滤泡性腺瘤组织和正常甲状腺组织(均P<0.05);甲状腺滤泡性腺瘤组织与正常甲状腺组织中HSP70、PPM1D、TNF-α阳性表达率比较均无统计学意义(均P>0.05)。TNM分期为Ⅲ~Ⅳ期、低分化、有淋巴结转移的患者甲状腺乳头状癌组织中HSP70、PPM1D、TNF-α阳性表达率均显著升高(均P<0.05)。结论HSP70、PPM1D、TNF-α表达与甲状腺乳头状癌的发生、发展有关,有望成为潜在的临床诊疗靶点。

Objective To investigate the expression of heat shock protein 70(HSP70),protein phosphatase magnesiumdependent 1 delta(PPM1D)and tumor necrosis factor-α(TNF-α)in papillary carcinoma of thyroid and their relationship with clinicopathological features.Method 92 thyroid histopathological specimens from Shenzhen People's Hospital from April 2016 to March 2019 were selected,including 45 thyroid papillary carcinoma tissues,32 thyroid follicular adenoma tissues and 15 normal thyroid tissues.The expressions of HSP70,PPM1D and TNF-αin the three groups were detected by immunohistochemistry.The relationship between HSP70,PPM1D and TNF-αand clinicopathological features were analyzed.Result The positive expression rates of HSP70,PPM1D and TNF-αin papillary carcinoma of thyroid were higher than those in thyroid follicular adenoma and normal thyroid tissue(all P<0.05),while the positive expression rates of HSP70,PPM1D and TNF-αin thyroid follicular adenoma tissues and normal thyroid tissues were not statistically significant(all P>0.05).Ⅲ~Ⅳperiod of TNM staging,low differentiation,lymph node metastasis in patients with papillary carcinoma of thyroid tissue of HSP70,PPM1D,TNF-αpositive expression rate were significantly increased(all P<0.05).Conclusion The expression of HSP70,PPM1D and TNF-αare related to the occurrence and develo pment of papillary carcinoma of thyroid,and are expected to be potential clinical targets.