定西市HIV/AIDS病人CD4^+T淋巴细胞检测分析

Analysis of detection for CD4^+T lymphocytes of HIV/AIDS patients in Dingxi city

目的了解甘肃省定西市艾滋病病人CD4^+T淋巴细胞水平和免疫状况,为判断艾滋病病人病程、预测临床进展和提高治疗效果提供科学依据。方法采用流式细胞术单平台法,检测HIV/AIDS病人外周静脉血液CD4^+T淋巴细胞并分析结果。结果2018年定西市共检测340例HIV/AIDS病人外周静脉血液标本,新发现病人CD4^+T淋巴细胞数最少29个/μl、最多676个/μl,≤200个/μl病人数最多、占35.09%,>500个/μl病人数最少、占10.53%;既往病人CD4^+T淋巴细胞数最少12个/μl、最多1375个/μl;接受治疗的病人治疗前后CD4^+T淋巴细胞≤200个/μl的构成比从18.37%降至7.76%(χ^2=12.149),351~500个/μl的构成比从46.53%降至23.26%(χ^2=29.185),>500个/μl的构成比从7.34%增至45.31%(χ^2=91.005),差异均有统计学意义(P<0.05);未接受治疗的病人首末次CD4^+T淋巴细胞检测数≤200个/μl的构成比从2.63%增至15.79%(χ^2=3.934),201~350个/μl的构成比从7.90%增至28.95%(χ^2=5.604),>500个/μl的构成比从50.00%降至18.42%(χ^2=8.418),差异均有统计学意义(P<0.05)。结论定西市部分HIV/AIDS病人发现较晚、免疫力较低,但抗病毒治疗效果较好,提示应采取有效措施做到早发现、早诊断、早治疗;应加大力度追踪随访CD4^+T淋巴细胞未检测或检测不及时的病人,动员其尽早检测和治疗。

Objective To understand the level of CD4^+T lymphocytes and immunization status of HIV/AIDS patients in Dingxi city,so as to provide scientific basis for estimating the clinical process of the patient and improving the treatment effect.Methods CD4^+T lymphocytes in peripheral venous blood of HIV/AIDS patients were detected by flow cytometer(FCM),and the results were analyzed.Results There were 340 peripheral venous blood samples of HIV/AIDS patients detected in Dingxi city,2018,with the minimum CD4^+T lymphocytes count 29 cells/μl and the maximum count 676 cells/μl in the newly found patients.The patients with CD4^+T lymphocytes count≤200 cells/μl were the most,accounting for35.09%,and the patients with CD4^+T lymphocytes count>500 cells/μl were the least,accounting for 10.53%.The minimum count of CD4^+T lymphocytes was 12 cells/μl and the maximum count was 1375 cells/μl in the previous cases.Out of the patients before and after treatment,the proportions decreased from 18.37%to 7.76%at a count≤200 cells/μl(χ^2=12.149),from 46.53%to 23.26%at the count 351-500 cells/μl(χ^2=29.185),and increased from 7.34%to 45.31%at the count>500 cells/μl(χ^2=91.005),with statistical differences(P<0.05).Among the patients without treatment,the proportions of the first and last detection for CD4^+T lymphocytes increased from 2.63%to 15.79%at the count≤200 cells/μl(χ^2=3.934),from 7.90%to 28.95%at the count 201-350 cells/μl(χ^2=5.604),and decreased from 50.00%to 18.42%at the count>500 cells/μl(χ^2=8.418),with statistical differences(P<0.05).Conclusions In Dingxi city,some HIV/AIDS patients are found late with low immunity,and the effect of antiviral treatment is good.It suggests that effective measures be taken for early discovery,diagnosis and treatment.The patients without detection or without prompt detection for CD4^+T lymphocytes should be tracked and followed further,and encouraged for detection and treatment as soon as possible.