TLR5 rs5744174基因多态性与肺炎链球菌肺炎的相关性

Association between TLR5 rs5744174 gene polymorphism and Streptococcus pneumoniae pneumonia

目的探讨Toll样受体5(TLR5)rs5744174基因多态性与肺炎链球菌肺炎的相关性。方法选取2014年1月至2018年10月本院收治的106例肺炎链球菌肺炎患者为观察组研究对象,同期选取85名健康体检者为对照组研究对象。收集受试者临床及病理相关资料,采用PCR结合测序法分析受试者TLR5 rs5744174基因多态性,分析肺炎链球菌肺炎患者支气管肺泡灌洗液(BALF)中细胞分类计数及C反应蛋白(CRP)水平与TLR5rs5744174基因多态性的关系。结果观察组与对照组年龄、吸烟、嗜酒及糖尿病比例等一般资料相比,差异有统计学意义(P<0.05)。观察组与对照组TLR5 rs5744174基因型分布均符合Hardy-Weinberg平衡定律检验水准(观察组χ^2=16.89,对照组χ^2=10.76,均P>0.05)。观察组与对照组TLR5 rs5744174(C0.05)。肺炎链球菌肺炎患者三种基因型(CC、CT、TT)间糖尿病比例比较,差异有统计学意义(P<0.05),支气管肺泡灌洗液(BALF)中性粒细胞百分比、CRP水平比较差异有统计学意义(P<0.05)。结论TLR5 rs5744174基因多态性与肺炎链球菌肺炎的发生可能不相关,与肺炎链球菌肺炎患者合并糖尿病比例、中性粒细胞百分比、CRP水平等水平有关,可能影响患者体内炎症反应程度。

Objective To investigate the correlation between TLR5 rs5744174 gene polymorphism and Streptococcus pneumoniae pneumonia.Methods One-hundred and six patients with Streptococcus pneumoniae pneumonia admitted to this hospital from January 2014 to October 2018 were selected as an observation group,and 85 healthy subjects were selected as a control group during the same period.The clinical and pathological data of the subjects were collected,polymorphism of TLR5 rs5744174 gene was analyzed by PCR and sequencing,and the relationships between cell classification count,C-reactive protein(CRP)level in bronchoalveolar lavage fluid(BALF)and TLR5 rs5744174 gene polymorphism in the patients with Streptococcus pneumoniae pneumonia were analyzed.Results There were significant differences in age,smoking,alcoholism,diabetes and the other general data between the observation group and the control group(P<0.05).The distribution of TLR5 rs5744174 genotype in the observation group and the control group was in accordance with Hardy-Weinberg equilibrium test level(χ^2=16.89 for the observation group,χ^2=10.76 for the control group,both P>0.05).There was no significant difference in the distribution frequency of TLR5 rs5744174(C<T)genotype and allele between the two groups(P>0.05).There were significant differences in the proportion of diabetes mellitus among the three genotypes(CC,CT,TT)of the patients with Streptococcus pneumoniae pneumonia(P<0.05).The percentage of neutrophils and CRP levels in BALF were significantly different(P<0.05).Conclusion The polymorphism of TLR5 rs5744174 gene may not be related to the occurrence of Streptococcus pneumoniae pneumonia,but is related to the proportion of complicated diabetes mellitus,the percentage of neutrophils and the level of CRP in patients with Streptococcus pneumoniae pneumonia,which may affect the degree of inflammation.