假基因ANXA2P1通过mTOR信号通路促进胶质瘤细胞生长

Pseudogene ANXA2P1 promotes glioma cell growth through mTOR signaling pathway

目的探讨ANXA2P1在神经胶质细胞瘤(GBM)组织中的表达及其在U87细胞中的生物学功能。方法通过TCGA数据库信息分析GBM组织中ANXA2P1的表达。利用细胞转染法上调或下调GBM组织或U87细胞中ANXA2P1的表达,通过CCK-8实验检测U87细胞的增殖能力,Western blotting实验检测U87细胞信号通路蛋白表达的变化。结果通过TCGA数据库分析发现,ANXA2P1在高级别的胶质瘤组织中表达水平明显高于低级别的胶质瘤组织(P<0.01)。在胶质瘤组织中ANXA2的表达水平与ANXA2P1的表达水平呈正相关(r=0.752,P<0.01)。过表达ANXA2P1的U87细胞增殖能力明显高于对照组,而Si-ANXA2P1 U87细胞的增殖能力明显低于对照组(P<0.05或P<0.01)。转染72 h后,过表达ANXA2P1细胞中p-S6K蛋白表达水平高于对照组(P<0.05),而Si-ANXA2P1细胞中p-S6K蛋白表达水平低于对照组(P<0.05)。结论ANXA2P1可能通过mTOR信号通路促进U87细胞的增殖。

Objective To investigate the expression of annexin A2 pseudogene 1(ANXA2P1)in glioblastoma(GBM)tissues and its biological function in U87 cells.Methods The expression level of ANXA2P1 in GBM tissues was analyzed by Cancer Genome Atlas(TCGA).Cell transfection was used to up-regulate or down-regulate the expression of ANXA2P1 in GBM tissues or U87 cells.Cell Counting Kit-8(CCK-8)was used to detect the proliferation ability of U87 cells.Western blotting was used to detect the changes of protein expression in signaling pathway of U87 cells.Results The TCGA analysis showed that the expression level of ANXA2P1 in high-level GBM tissues was significantly higher than that in low-level GBM tissues(P<0.01).In GBM tissues,the expression level of ANXA2 was positively correlated with that of ANXA2P1(r=0.752,P<0.01).The proliferation capacity of U87 cells with ANXA2P1 overexpression was higher than that of the control group,while the proliferation capacity of U87 cells transfected by Si-ANXA2P1 was lower than that of the control group.At 72 h after transfection,the expression level of p-S6K protein in the ANXA2PI-overexpressed cells was higher than that in the control group(P<0.05),while the expression level of p-S6K protein in the Si-ANXA2P1 cells was lower than that in the control group(P<0.05).Conclusion ANXA2P1 may promote the proliferation of U87 cells through mTOR signaling pathway.