八种常用农药与蜡蚧轮枝菌JMC-01的相容性及对烟粉虱若虫的毒力测定

Compatibility of 8 common pesticides with Lecanicillium lecanii JMC-01 and the toxicity of different pesticide-L.lecanii combinations toxicity to Bemisia tabaci nymphs

【目的】为明确8种常用农药与蜡蚧轮枝菌的相容性及对烟粉虱Bemisia tabaci若虫的毒力。【方法】该试验采用涂板法和浸渍法分别测定了8种常用农药对蜡蚧轮枝菌菌丝生长、孢子萌发和产孢量以及对烟粉虱若虫的LC50和共毒系数。【结果】结果表明,随着农药稀释倍数的增加,蜡蚧轮枝菌菌丝生长抑制率、孢子萌发抑制率和产孢抑制率依次降低。其中,生物源农药乙基多杀菌素、藜芦碱、印楝素与蜡蚧轮枝菌的相容性和协同增效作用最好。乙基多杀菌素、藜芦碱、印楝素在10倍稀释浓度下,孢子萌发抑制率分别为20.02%、16.41%、15.38%;产孢抑制率分别为17.77%、15.90%、14.96%;菌丝生长抑制率分别为9.96%、8.87%、9.74%。蜡蚧轮枝菌与印楝素、藜芦碱混合后协同增效作用最好,CTC最大值分别为315和302。【结论】因此,应用蜡蚧轮枝菌防治烟粉虱,选择相容性好、共毒系数高的农药以低剂量与蜡蚧轮枝菌复配使用,可以大幅度减少农药的用量,对温室大棚烟粉虱的生物防治提供参考依据。

[Objectives]To determine the compatibility of eight common pesticides with Lecanicillium lecanii JMC-01,and the toxicity of combinations of each pesticide and L.lecanii JMC-01 to Bemisia tabaci nymphs.[Methods]The effects of each of eight pesticides on the spore germination and sporulation of L.lecanii,and their LC50 and co-toxicity coefficients with respect to B.tabaci nymphs,were tested using the coating and dipping methods.[Results]The growth inhibition rate,spore germination inhibition rate and sporulation inhibition rate of L.lecanii decreased with pesticide dilution factor.The bio-pesticide lspinetoram,veratrine and azadirachtin were the most compatibile and had the best synergistic effect with L.lecanii.The inhibition rates of spore germination for 10-fold dilutions of lspinetoram,veratrine and azadirachtin were 20.02%,16.41%,and 15.38%,respectively,and sporulation inhibition rates were 17.77%,15.90%,and 14.96%,respectively.Mycelial growth inhibition rates for the above pesticides were 9.96%,8.87%,and 9.74%,respectively.The best synergistic control was achieved using a combination of azadirachtin and veratrine with L.lecanii.Maximum CTC was 315 and 302,respectively.[Conclusion]Selecting pesticides that are compatibile with L.lecanii,and that have a high co-toxicity coefficient at low doses,can significantly reduce the amount of pesticide required to control B.tabaci in greenhouses.