昆虫共生菌对宿主功能研究的方法体系

Review of the methodology used in the functional analysis of insect-microbial symbioses

共生菌可参与昆虫的生理生化过程,影响昆虫的营养、生长发育、解毒作用、天敌防御和免疫能力等。在共生菌对宿主功能的研究中,核心问题是如何从组成复杂的共生菌组中筛选出具有特定功能的共生菌。共生菌对宿主功能的研究模式通常包括:通过共生菌多样性分析,提出差异共生菌对宿主功能的假设;分析并验证特定共生菌在宿主体内的功能。本文围绕共生菌对宿主功能的研究模式,系统地总结和比较昆虫共生菌功能研究的方法和技术,构建昆虫对宿主功能研究的方法体系,以期推进共生菌-宿主联系的研究。

Microbial symbionts play an important part in the physiological and biochemical processes of insects,and can affect the nutrition,development,insecticide resistance,predator defense and immunity of their hosts.The key question in research on the function of insect-microbial symbioses is how to isolate symbiotic microbes with specific functions from the complex symbiotic microbiome.Identifying the function of insect-microbial symbioses typically involves proposing hypotheses for the function of certain symbionts based on a diversity analysis of microbiomes from different host phenotypes,then analyzing and verifying the function of certain symbionts.This review systematically summarizes and compares various technologies used in function analysis of insect-microbial symbioses,and constructs a general methodology for this kind of research to accelerate the study of interactions between insects and their microbial symbionts.