血管生成因子A抑制剂对子宫内膜异位症模型大鼠干预效果及作用机制研究

The effect and mechanism of vascular endothelial growth factor-A inhibitor for intervening model rats with endometriosis

目的:探究血管生成因子A(VEGF-A)抑制剂对子宫内膜异位症模型大鼠的干预效果及作用机制。方法:选取35只SD健康雌性大鼠,正常大鼠组5只,余30只建立子宫内膜异位症模型并分别干预(模型组、丹那唑组、VEGF-A抑制剂组各10只)。检测各组雌二醇(E2)、孕酮(P)、卵泡刺激素(FSH)、促黄体生成激素(LH)、C反应蛋白(CRP)、白细胞介素-8(IL-8)、IL-6水平,Western blot法检测缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)、Bcl-2、Bax、半胱氨酸天冬氨酸蛋白酶3(caspase3)蛋白表达。结果:VEGF-A抑制剂组E2、P、FSH、LH水平,CRP、IL-8、IL-6水平,VEGF、HIF-1α及Bcl-2、caspase3相对表达量均低于丹那唑组、模型组,Bax相对表达量高于丹那唑组、模型组(均P<0.05)。结论:使用VEGF-A抑制剂干预子宫内膜异位症模型大鼠,能够调控其性激素水平,抑制炎症反应和异位内膜组织生长,促进异位内膜组织凋亡。

Objective:To explore the effect and mechanism of vascular endothelial growth factor-A(VEGF-A) inhibitor intervening model rats with endometriosis. Methods: 35 SD healthy female rats were selected and divided in 2 groups, which included 5 rats in the normal group and 30 rats were in the endometriosis model group. And the 30 rats in the endometriosis model group were randomly divided into group A(10 rats were given saline), group B(10 rats were given danazol), and group C(10 rats were given VEGF-A inhibitor). The levels of estradiol(E2), progesterone(P), follicle stimulating hormone(FSH), luteinizing hormone(LH), C-reactive protein(CRP), interleukin-8(IL-8), interleukin-6(IL-6) of rates in the four groups were detected, and the levels of Hypoxia inducible factor-1α(HIF-1α) and VEGF, and the expression of Bcl-2, Bax, caspase-3 proteins of rates in the four groups were detected by Western blot. Results: The levels of E2, P, FSH, LH, CRP, IL-8 and IL-6, and the relative expression of VEGF, HIF-1α, Bcl-2 and Caspase-3 of rats in group C were significant lower than those in group A and B, but the relative expression level of Bax of rats in group C was significant higher(P<0.05). Conclusion: VEGF-A inhibitors used for intervening the model rats with endometriosis can regulate their sex hormones level, inhibit the inflammation and growth of heterotopic endometrium tissue, and promote apoptosis of ectopic endometrium tissue.