摘要
介绍了影响层析介质分离纯化效果的结构参数。总结了测定色谱介质孔径结构常用的方法,如压汞法、氮气吸附法、小角X射线散射法、电镜观察法(SEM和TEM)和逆体积排阻色谱法(ISEC)。介绍了ISEC的基本原理及其涉及到的探针类型。对ISEC相关理论,尤其是溶质半径、色谱理论和体积排阻色谱法(SEC)随机理论进行了重点阐述。比较了ISEC的优缺点。介绍了ISEC在评价介质孔径结构中的应用,对ISEC的应用前景进行了展望。
The structural parameters affecting the efficiency of separation and purification of chromatography media are introduced.Several common methods to determine pore structure of chromatographic media,such as mercury intrusion porosimetry,nitrogen adsorption method,small angle X-ray scattering,electron microscopies(TEM and SEM),and inverse size-exclusion chromatography(ISEC)are summarized.The basic principle of ISEC and its involved probe types are described.The related theories of ISEC,especially solute radius,chromatographic theory and size-exclusion chromatography(SEC)random theory are discussed.The advantages and disadvantages of ISEC are compared.The application of ISEC in evaluating pore size structure of chromatographic media is introduced.The potential application of ISEC is prospected.
作者
池伟亚
侯恒磊
康红艳
齐莉
靳海波
张荣月
CHI Weiya;HOU Henglei;KANG Hongyan;QI Li;JIN Haibo;ZHANG Rongyue(Beijing Key Laboratory of Fuel Cleanliness and Efficient Catalytic Emission Reduction Technology,School of Chemical Engineering,Beijing Instute of Petrochemical Technology,Beijing 102617,China;Clinical Laboratory,The Eighth Hospital of Tangshan,Tangshan 063000,Hebei,China;Key Laboratory of Living Body Analytical Chemistry Institute,Institute of Chemistry Chinese Academy of Sciences,Beijing 100190,China)
出处
《精细化工》
EI
CAS
CSCD
北大核心
2020年第7期1320-1326,1428,共8页
Fine Chemicals
基金
北京市委组织部青年拔尖人才项目(ZZB2019001)
北京市属高校高水平教师队伍建设支持计划高水平创新团队建设计划项目(IDHT20180508)。
关键词
逆体积排阻色谱法
层析介质
孔径结构
测定方法
inverse size-exclusion chromatography
chromatography media
pore structure
determination method
