香根草精油微胶囊的制备及其体外抗炎作用

Preparation of vetiver essential oil microcapsules and its anti-inflammatory effect in vitro

以明胶和壳聚糖为壁层材料,以香根草精油为囊芯,采用复凝聚法制备香根草精油微胶囊。通过单因素和正交实验筛选微胶囊最佳制备工艺,采用SEM、粒度仪、Zeta电位仪和FTIR对样品进行了表征。测定微胶囊于pH 1.5和7.4时香根草精油的释放率,观察微胶囊对小鼠RAW 264.7细胞的体外抗炎作用。结果表明,香根草精油用量0.3 g,壁材用量1.0 g(其中明胶与壳聚糖的质量比为30∶2)时,封装率可达90.96%;制备的微胶囊表面呈球形,微胶囊粒径为2.303μm,电位–33.8 mV,具有良好的稳定性,封装效果好;该微胶囊于pH 7.4下释放度较佳,72 h内累积释放达87.49%;细胞实验表明,该微胶囊(质量浓度为60、90、120 mg/L)安全无毒,能有效抑制RAW 264.7细胞白介素-1β(IL-1β)、白介素-6(IL-6)和肿瘤坏死因子α(TNF-α)的分泌。香根草精油微胶囊处方工艺简单,质量稳定,抗炎活性良好。

Vetiver essential oil microcapsules were prepared using gelatin and chitosan as the shell material and vetiver essential oil as the core.The best preparation process of microcapsules was optimaized by single factor and orthogonal experiments.The samples were characterized by SEM,particle size analyzer,Zeta potential instrument and FTIR.The release of vetiver essential oil in microcapsules at pH 1.5 and 7.4 was measured,and the anti-inflammatory effect of microcapsules in vitro was studied by mouse RAW 246.7 cells.The results showed that the encapsulation rate reached 90.96%under the conditions of vetiver essential oil dosage 0.3 g,wall material dosage 1.0 g〔m(gelatin)∶m(chitosan)〕=30∶2.The surface of the microcapsules was continuous and spherical.The particle size of the microcapsules was 2.303μm and the Zeta potential was–33.8 mV.The prepared microcapsules had good stability and excellent packaging effect.The microcapsules showed a good release character at pH 7.4 with a cumulative release of 87.49%within 72 h.Cell experiments revealed that the microcapsules(60,90,120 mg/L)were safe and non-toxic,which couldd effectively inhibit the interleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor alpha(TNF-α)secretion.The vetiver essential oil microcapsules are simple to prepare and stable in quality with good anti-inflammatory activity.